KeyTec® AAK1, N-His (E.coli)

Used for Developing AAK1 Biochemical Activity Assay or Binding Assay Models
•  Strict quality control: Each batch comes with a rigorous QC report
•  High activity: Each batch is activity-verified, providing high-quality protein
•  Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Size: 10 μg100 μg

Price: $ 660

CAT.: P1HE0001S

Delivery: 2-3 weeks

KeyTec® AAK1, N-His (E.coli)

Used for Developing AAK1 Biochemical Activity Assay or Binding Assay Models

• Strict quality control: Each batch comes with a rigorous QC report • High activity: Each batch is activity-verified, providing high-quality protein • Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Size: 10 μg100 μg

CAT.: P1HE0001S

Delivery: 2-3 weeks

Price: $ 660

Overview

KeyTec® AAK1, N-His (E.coli) recombinant protein with N-terminal 6xHis tag was purified by Ni-NTA affinity and followed by SEC chromatography. The SEC-HPLC result showed that the AAK1 protein was dimer and monomer in the solution. The AAK1 protein showed high activity in ADP-Glo assay.
AA Sequences：Uniprot: Q2M2I8-1;T27-A365
Tag：N-terminal 6xHis tag
Molecular Weight：40.2 kDa
Species：Human
Expression Host：E.coli
Protein Concentration：0.32 mg/mL by OD280
Purity：> 95% by SDS-PAGE
Form：Liquid
Formulation：20 mM Tris, 300 mM NaCl, 1 mM DTT, 10% glycerol, pH 7.5

Performance

The AAK1 activity was detected using ADP-Glo technology. The reaction was performed by incubating the AAK1 protein, ATP and substrate at 25 ℃ for 120 min, then reading RLU by Microplate Reader with Luminescence.

Protocols

COA_P1HE0001_A121024011_EN.pdf

Components

CAT.

Description

Size

P1HE0001S

KeyTec® AAK1, N-His (E.coli)

10 μg

Notices

Certificate of Analysis

Limitations

Storage Conditions

For research use only

-80 ℃

Related Products

Overview

KeyTec® AAK1, N-His (E.coli) recombinant protein with N-terminal 6xHis tag was purified by Ni-NTA affinity and followed by SEC chromatography. The SEC-HPLC result showed that the AAK1 protein was dimer and monomer in the solution. The AAK1 protein showed high activity in ADP-Glo assay.
AA Sequences：Uniprot: Q2M2I8-1;T27-A365
Tag：N-terminal 6xHis tag
Molecular Weight：40.2 kDa
Species：Human
Expression Host：E.coli
Protein Concentration：0.32 mg/mL by OD280
Purity：> 95% by SDS-PAGE
Form：Liquid
Formulation：20 mM Tris, 300 mM NaCl, 1 mM DTT, 10% glycerol, pH 7.5

Performance

The AAK1 activity was detected using ADP-Glo technology. The reaction was performed by incubating the AAK1 protein, ATP and substrate at 25 ℃ for 120 min, then reading RLU by Microplate Reader with Luminescence.

Protocols

COA_P1HE0001_A121024011_EN.pdf

Components

CAT.

Description

Size

P1HE0001S

KeyTec® AAK1, N-His (E.coli)

10 μg

Notices

Certificate of Analysis

Limitations