KeyTec® CDK1/CycA2, N-GST/N-GST;C-His

    Used for Developing CDK1/CycA2 Biochemical Activity Assay or Binding Assay Models

    • Strict quality control: Each batch comes with a rigorous QC report • High activity: Each batch is activity-verified, providing high-quality protein • Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
Size: 10 μg100 μg
Price: $ 660
CAT.: P1HI0105S
Delivery: 2-3 weeks
KeyTec® CDK1/CycA2, N-GST/N-GST;C-His

    Used for Developing CDK1/CycA2 Biochemical Activity Assay or Binding Assay Models

    • Strict quality control: Each batch comes with a rigorous QC report • High activity: Each batch is activity-verified, providing high-quality protein • Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
Size: 10 μg100 μg
CAT.: P1HI0105S
Delivery: 2-3 weeks
Price: $ 660
Overview
KeyTec® CDK1/CycA2, N-GST/N-GST;C-His recombinant protein was co-expressed recombinant human CDK1 with N-terminal GST tag, CycA2 protein with N-terminal GST tag and C- terminal 8x His tag. Recombinant human CDK1/CycA2 protein was purified by GST affinity and followed by SEC chromatography. The CDK1/CycA2 protein showed high activity in ADP-Glo assay. AA Sequences: Uniprot: CDK1: P06493-1, M1-M297(end); CycA2: P20248, E174-L432(end) Tag: CDK1: N-terminal GST tag; CycA2: N-terminal GST tag, C-terminal 8x His tag Molecular Weight: CDK1: 60.7 kDa; CycA2: 56.8 kDa Species: Human Expression Host: Sf9 Protein Concentration: 0.99 mg/mL by OD280 Purity: >95% by SDS-PAGE Form: Liquid Formulation: 50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, 0.05% Brij35, pH7.5
Performance
The CDK1/CycA2 activity was detected using ADP-Glo technology. The reaction was performed by incubating the CDK1/CycA2 protein, ATP and substrate at 25 ℃ for 60 min, then reading RLU by Microplate Reader with Luminescence.
Protocols
COA_P1HI0105_K130801012_EN.pdf
Components
CAT.
Description
Size
P1HI0105S
KeyTec® CDK1/CycA2, N-GST/N-GST;C-His
10 μg
Notices
Certificate of Analysis
Limitations
Storage Conditions
For research use only

-80 ℃

Related Products
Overview
KeyTec® CDK1/CycA2, N-GST/N-GST;C-His recombinant protein was co-expressed recombinant human CDK1 with N-terminal GST tag, CycA2 protein with N-terminal GST tag and C- terminal 8x His tag. Recombinant human CDK1/CycA2 protein was purified by GST affinity and followed by SEC chromatography. The CDK1/CycA2 protein showed high activity in ADP-Glo assay. AA Sequences: Uniprot: CDK1: P06493-1, M1-M297(end); CycA2: P20248, E174-L432(end) Tag: CDK1: N-terminal GST tag; CycA2: N-terminal GST tag, C-terminal 8x His tag Molecular Weight: CDK1: 60.7 kDa; CycA2: 56.8 kDa Species: Human Expression Host: Sf9 Protein Concentration: 0.99 mg/mL by OD280 Purity: >95% by SDS-PAGE Form: Liquid Formulation: 50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, 0.05% Brij35, pH7.5
Performance
The CDK1/CycA2 activity was detected using ADP-Glo technology. The reaction was performed by incubating the CDK1/CycA2 protein, ATP and substrate at 25 ℃ for 60 min, then reading RLU by Microplate Reader with Luminescence.
Protocols
COA_P1HI0105_K130801012_EN.pdf
Components
CAT.
Description
Size
P1HI0105S
KeyTec® CDK1/CycA2, N-GST/N-GST;C-His
10 μg
Notices
Certificate of Analysis
Limitations
Storage Conditions
For research use only

-80 ℃

Related Products

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