KeyTec® CDK1/CycB1, N-GST/N-GST
Used for Developing CDK1/CycB1 Biochemical Activity Assay or Binding Assay Models
• Strict quality control: Each batch comes with a rigorous QC report • High activity: Each batch is activity-verified, providing high-quality protein • Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
Size:
10 μg100 μg
Price: $ 660
CAT.: P1HI0015S
Delivery: 2-3 weeks
KeyTec® CDK1/CycB1, N-GST/N-GST
Used for Developing CDK1/CycB1 Biochemical Activity Assay or Binding Assay Models
• Strict quality control: Each batch comes with a rigorous QC report • High activity: Each batch is activity-verified, providing high-quality protein • Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
Size:
10 μg100 μg
CAT.: P1HI0015S
Delivery: 2-3 weeks
Price: $ 660
OverviewKeyTec® CDK1/CycB1, N-GST/N-GST recombinant protein was co-expressed recombinant human CDK1 with N-terminal GST tag and CycB1 protein with N-terminal GST tag. The complex was purified by GST affinity. The CDK1/CycB1 protein showed high activity in ADP-Glo assay.
AA Sequences： Uniprot: CDK1: P06493-1, M1-M297(end); CycB1: P14635-1, M1-V433
Tag： CDK1: N-terminal GST tag; CycB1: N-terminal GST tag
Molecular Weight： CDK1: 60.7 kDa; CycB1: 74.9 kDa
Species： Human
Expression Host： Sf9
Protein Concentration： 0.57 mg/mL by OD280
Purity： > 85% by SDS-PAGE
Form： Liquid
Formulation： 50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, 0.05% Brij35, PH7.5
Performance
The CDK1/CycB1 activity was detected using ADP-Glo technology. The reaction was performed by incubating the CDK1/CycB1 protein, ATP and substrate at 25 ℃ for 60 min, then reading RLU by Microplate Reader with Luminescence.
Protocols
COA_P1HI0015_G130517021_EN.pdf
ComponentsCAT.
Description
Size
P1HI0015S
KeyTec® CDK1/CycB1, N-GST/N-GST
10 μg
NoticesCertificate of Analysis
Limitations
Storage Conditions
For research use only
-80 ℃
Related ProductsOverviewKeyTec® CDK1/CycB1, N-GST/N-GST recombinant protein was co-expressed recombinant human CDK1 with N-terminal GST tag and CycB1 protein with N-terminal GST tag. The complex was purified by GST affinity. The CDK1/CycB1 protein showed high activity in ADP-Glo assay.
AA Sequences： Uniprot: CDK1: P06493-1, M1-M297(end); CycB1: P14635-1, M1-V433
Tag： CDK1: N-terminal GST tag; CycB1: N-terminal GST tag
Molecular Weight： CDK1: 60.7 kDa; CycB1: 74.9 kDa
Species： Human
Expression Host： Sf9
Protein Concentration： 0.57 mg/mL by OD280
Purity： > 85% by SDS-PAGE
Form： Liquid
Formulation： 50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, 0.05% Brij35, PH7.5
PerformanceThe CDK1/CycB1 activity was detected using ADP-Glo technology. The reaction was performed by incubating the CDK1/CycB1 protein, ATP and substrate at 25 ℃ for 60 min, then reading RLU by Microplate Reader with Luminescence.
ProtocolsCOA_P1HI0015_G130517021_EN.pdf
ComponentsCAT.
Description
Size
P1HI0015S
KeyTec® CDK1/CycB1, N-GST/N-GST
10 μg
NoticesCertificate of Analysis
Limitations
Storage Conditions
For research use only
-80 ℃
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