KeyTec® CK1ε(CSNK1E), N-His
Used for Developing CK1ε(CSNK1E) Biochemical Activity Assay or Binding Assay Models
• Strict quality control: Each batch comes with a rigorous QC report • High activity: Each batch is activity-verified, providing high-quality protein • Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
Size:
10 μg100 μg
Price: $ 660
CAT.: P1HE0112S
Delivery: 2-3 weeks
KeyTec® CK1ε(CSNK1E), N-His
Used for Developing CK1ε(CSNK1E) Biochemical Activity Assay or Binding Assay Models
• Strict quality control: Each batch comes with a rigorous QC report • High activity: Each batch is activity-verified, providing high-quality protein • Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
Size:
10 μg100 μg
CAT.: P1HE0112S
Delivery: 2-3 weeks
Price: $ 660
OverviewKeyTec® CK1ε(CSNK1E), N-His recombinant protein with N-terminal 8xHis tag was purified by Ni-NTA affinity chromatography. The CK1ε(CSNK1E) protein showed high activity in ADP-Glo assay.
AA Sequences： Uniprot: P49674, M1-K294
Tag： N-terminal 8x His tag
Molecular Weight： 35.6 kDa
Species： Human
Expression Host： Sf9
Protein Concentration： 0.12 mg/mL by OD280
Purity：> 95% by SDS-PAGE
Form： Liquid
Formulation： 50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5
Performance
The CK1ε(CSNK1E) activity was detected using ADP-Glo technology. The reaction was performed by incubating the CK1ε(CSNK1E) protein, ATP and substrate at 25 ℃ for 60 min, then reading RLU by Microplate Reader with Luminescence.
Protocols
COA_P1HE0112_A130725012_EN.pdf
ComponentsCAT.
Description
Size
P1HE0112S
KeyTec® CK1ε(CSNK1E), N-His
10 μg
NoticesCertificate of Analysis
Limitations
Storage Conditions
For research use only
-80 ℃
Related ProductsOverviewKeyTec® CK1ε(CSNK1E), N-His recombinant protein with N-terminal 8xHis tag was purified by Ni-NTA affinity chromatography. The CK1ε(CSNK1E) protein showed high activity in ADP-Glo assay.
AA Sequences： Uniprot: P49674, M1-K294
Tag： N-terminal 8x His tag
Molecular Weight： 35.6 kDa
Species： Human
Expression Host： Sf9
Protein Concentration： 0.12 mg/mL by OD280
Purity：> 95% by SDS-PAGE
Form： Liquid
Formulation： 50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5
PerformanceThe CK1ε(CSNK1E) activity was detected using ADP-Glo technology. The reaction was performed by incubating the CK1ε(CSNK1E) protein, ATP and substrate at 25 ℃ for 60 min, then reading RLU by Microplate Reader with Luminescence.
ProtocolsCOA_P1HE0112_A130725012_EN.pdf
ComponentsCAT.
Description
Size
P1HE0112S
KeyTec® CK1ε(CSNK1E), N-His
10 μg
NoticesCertificate of Analysis
Limitations
Storage Conditions
For research use only
-80 ℃
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