KeyTec® EGFR, N-His

Used for Developing EGFR Biochemical Activity Assay or Binding Assay Models
•  Strict quality control: Each batch comes with a rigorous QC report
•  High activity: Each batch is activity-verified, providing high-quality protein
•  Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Size: 10 μg100 μg

Price: $ 660

CAT.: P1HI0032S

Delivery: 2-3 weeks

KeyTec® EGFR, N-His

Used for Developing EGFR Biochemical Activity Assay or Binding Assay Models

• Strict quality control: Each batch comes with a rigorous QC report • High activity: Each batch is activity-verified, providing high-quality protein • Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Size: 10 μg100 μg

CAT.: P1HI0032S

Delivery: 2-3 weeks

Price: $ 660

Overview

KeyTec® EGFR, N-His recombinant protein with N-terminal 6xHis tag + TEV cleavage site was purified by Ni-NTA affinity chromatography. The EGFR protein showed high activity in TR-FRET assay.
AA Sequences：	Uniprot: P00533-1; H672-Y998
Tag：		N-terminal 6x His tag
Molecular Weight：		39.5 kDa
Species：	Human
Expression Host：	Sf9
Protein Concentration：	0.55 mg/mL by OD280
Purity：> 80% by SDS-PAGE
Form：	Liquid
Formulation：	20 mM Hepes, 300 mM NaCl, 1 mM DTT, 10% Glycerol, pH7.5

Performance

The EGFR activity was detected using TR-FRET technology. The reaction was performed by incubating the EGFR protein, ATP and substrate at 25 ℃ for 60 min, adding the TR-FRET reagents at 25℃ for 60 min, then reading ratio 665/620 by Microplate Reader with TR-FRET.

Protocols

COA_P1HI0032_K121222021_EN.pdf

Components

CAT.

Description

Size

P1HI0032S

KeyTec® EGFR, N-His

10 μg

Notices

Certificate of Analysis

Limitations

Storage Conditions

For research use only

-80 ℃

Related Products

Overview

KeyTec® EGFR, N-His recombinant protein with N-terminal 6xHis tag + TEV cleavage site was purified by Ni-NTA affinity chromatography. The EGFR protein showed high activity in TR-FRET assay.
AA Sequences：	Uniprot: P00533-1; H672-Y998
Tag：		N-terminal 6x His tag
Molecular Weight：		39.5 kDa
Species：	Human
Expression Host：	Sf9
Protein Concentration：	0.55 mg/mL by OD280
Purity：> 80% by SDS-PAGE
Form：	Liquid
Formulation：	20 mM Hepes, 300 mM NaCl, 1 mM DTT, 10% Glycerol, pH7.5

Performance

The EGFR activity was detected using TR-FRET technology. The reaction was performed by incubating the EGFR protein, ATP and substrate at 25 ℃ for 60 min, adding the TR-FRET reagents at 25℃ for 60 min, then reading ratio 665/620 by Microplate Reader with TR-FRET.

Protocols

COA_P1HI0032_K121222021_EN.pdf

Components

CAT.

Description

Size

P1HI0032S

KeyTec® EGFR, N-His

10 μg

Notices

Certificate of Analysis

Limitations