KeyTec® EPHB2, N-GST recombinant protein with N-terminal GST tag + TEV cleavage site was purified by GST affinity and followed by SEC chromatography. The SEC-HPLC result showed that the EPHB2 protein was trimer in the solution. The EPHB2 protein showed high activity in TR-FRET assay.
AA Sequences: Uniprot: Q6NVW1, K581-V987
Tag: N-terminal GST tag
Molecular Weight: 72.7 kDa
Species: Human
Expression Host: Sf9
Protein Concentration: 1.06 mg/mL by OD280
Purity:>95% by SDS-PAGE
Form: Liquid
Formulation: 50mM Tris, 150mM NaCl, 20mM Reduced Glu, 1mM DTT, 10% Glycerol, 0.05% Brij-35, pH7.5