KeyTec® FGFR4[V550L], N-GST

    Used for Developing FGFR4[V550L] Biochemical Activity Assay or Binding Assay Models

    • Strict quality control: Each batch comes with a rigorous QC report • High activity: Each batch is activity-verified, providing high-quality protein • Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
Size: 10 μg100 μg
Price: $ 945
CAT.: P1HI0062S
Delivery: 2-3 weeks
KeyTec® FGFR4[V550L], N-GST

    Used for Developing FGFR4[V550L] Biochemical Activity Assay or Binding Assay Models

    • Strict quality control: Each batch comes with a rigorous QC report • High activity: Each batch is activity-verified, providing high-quality protein • Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
Size: 10 μg100 μg
CAT.: P1HI0062S
Delivery: 2-3 weeks
Price: $ 945
Overview
KeyTec® FGFR4[V555L], N-GST recombinant protein with N-terminal GST tag + TEV cleavage site was purified by GST affinity chromatography. The FGFR4[V555L] protein showed high activity in TR-FRET assay. AA Sequences: Uniprot: P22455-1, W460-T802(end), V550L Tag: N-terminal GST tag Molecular Weight: 64.6 kDa Species: Human Expression Host: Sf9 Protein Concentration: 0.83 mg/mL by OD280 Purity:> 90% by SDS-PAGE Form: Liquid Formulation: 50 mM Tris, 150 mM NaCl, 1 mM DTT, 20 mM reduced glutathione, 10% glycerol, 0.05% Brij35, pH7.5
Performance
The FGFR4[V555L] activity was detected using TR-FRET technology. The reaction was performed by incubating the FGFR4[V555L] protein, ATP and substrate at 25 ℃ for 60 min, adding the TR-FRET reagents at 25 ℃ for 60 min, then reading ratio 665/620 by Microplate Reader with TR-FRET.
Protocols
COA_P1HI0062_G130712011_EN.pdf
Components
CAT.
Description
Size
P1HI0062S
KeyTec® FGFR4[V550L], N-GST
10 μg
Notices
Certificate of Analysis
Limitations
Storage Conditions
For research use only

-80 ℃

Related Products
Overview
KeyTec® FGFR4[V555L], N-GST recombinant protein with N-terminal GST tag + TEV cleavage site was purified by GST affinity chromatography. The FGFR4[V555L] protein showed high activity in TR-FRET assay. AA Sequences: Uniprot: P22455-1, W460-T802(end), V550L Tag: N-terminal GST tag Molecular Weight: 64.6 kDa Species: Human Expression Host: Sf9 Protein Concentration: 0.83 mg/mL by OD280 Purity:> 90% by SDS-PAGE Form: Liquid Formulation: 50 mM Tris, 150 mM NaCl, 1 mM DTT, 20 mM reduced glutathione, 10% glycerol, 0.05% Brij35, pH7.5
Performance
The FGFR4[V555L] activity was detected using TR-FRET technology. The reaction was performed by incubating the FGFR4[V555L] protein, ATP and substrate at 25 ℃ for 60 min, adding the TR-FRET reagents at 25 ℃ for 60 min, then reading ratio 665/620 by Microplate Reader with TR-FRET.
Protocols
COA_P1HI0062_G130712011_EN.pdf
Components
CAT.
Description
Size
P1HI0062S
KeyTec® FGFR4[V550L], N-GST
10 μg
Notices
Certificate of Analysis
Limitations
Storage Conditions
For research use only

-80 ℃

Related Products

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