KeyTec® FLT3-ITD, N-GST; C-His
Used for Developing FLT3-ITD Biochemical Activity Assay or Binding Assay Models
• Strict quality control: Each batch comes with a rigorous QC report • High activity: Each batch is activity-verified, providing high-quality protein • Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
Size:
10 μg100 μg
Price: $ 660
CAT.: P1HI0146S
Delivery: 2-3 weeks
KeyTec® FLT3-ITD, N-GST; C-His
Used for Developing FLT3-ITD Biochemical Activity Assay or Binding Assay Models
• Strict quality control: Each batch comes with a rigorous QC report • High activity: Each batch is activity-verified, providing high-quality protein • Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
Size:
10 μg100 μg
CAT.: P1HI0146S
Delivery: 2-3 weeks
Price: $ 660
OverviewKeyTec® FLT3-ITD, N-GST; C-His recombinant protein with N-terminal GST tag + 3C cleavage site and C-terminal 6xHis tag was purified by Ni-NTA affinity chromatography. The FLT3-ITD protein showed high activity in TR-FRET assay.
AA Sequences：Uniprot: P36888-1, R571-S993
Tag： N-terminal GST tag, C-terminal 6x His tag
Molecular Weight： 77.8 kDa
Species：Human
Expression Host：Sf9
Protein Concentration：0.24 mg/mL by OD280
Purity：>95% by SDS-PAGE
Form：Liquid
Formulation：50 mM Tris, 150 mM NaCl, 1 mM DTT, pH7.5
Performance
The FLT3-ITD activity was detected using TR-FRET technology. The reaction was performed by incubating the FLT3-ITD protein, ATP and substrate at 25 ℃ for 60 min, adding the TR-FRET reagents at 25 ℃ for 60 min, then reading ratio 665/620 by Microplate Reader with TR-FRET.
Protocols
COA_P1HI0146_K130824011_EN.pdf
ComponentsCAT.
Description
Size
P1HI0146S
KeyTec® FLT3-ITD, N-GST; C-His
10 μg
NoticesCertificate of Analysis
Limitations
Storage Conditions
For research use only
-80 ℃
Related ProductsOverviewKeyTec® FLT3-ITD, N-GST; C-His recombinant protein with N-terminal GST tag + 3C cleavage site and C-terminal 6xHis tag was purified by Ni-NTA affinity chromatography. The FLT3-ITD protein showed high activity in TR-FRET assay.
AA Sequences：Uniprot: P36888-1, R571-S993
Tag： N-terminal GST tag, C-terminal 6x His tag
Molecular Weight： 77.8 kDa
Species：Human
Expression Host：Sf9
Protein Concentration：0.24 mg/mL by OD280
Purity：>95% by SDS-PAGE
Form：Liquid
Formulation：50 mM Tris, 150 mM NaCl, 1 mM DTT, pH7.5
PerformanceThe FLT3-ITD activity was detected using TR-FRET technology. The reaction was performed by incubating the FLT3-ITD protein, ATP and substrate at 25 ℃ for 60 min, adding the TR-FRET reagents at 25 ℃ for 60 min, then reading ratio 665/620 by Microplate Reader with TR-FRET.
ProtocolsCOA_P1HI0146_K130824011_EN.pdf
ComponentsCAT.
Description
Size
P1HI0146S
KeyTec® FLT3-ITD, N-GST; C-His
10 μg
NoticesCertificate of Analysis
Limitations
Storage Conditions
For research use only
-80 ℃
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