KeyTec® GSK3β, N-GST
Used for Developing GSK3β Biochemical Activity Assay or Binding Assay Models
• Strict quality control: Each batch comes with a rigorous QC report • High activity: Each batch is activity-verified, providing high-quality protein • Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
Size:
10 μg100 μg
Price: $ 660
CAT.: P1HI0151S
Delivery: 2-3 weeks
KeyTec® GSK3β, N-GST
Used for Developing GSK3β Biochemical Activity Assay or Binding Assay Models
• Strict quality control: Each batch comes with a rigorous QC report • High activity: Each batch is activity-verified, providing high-quality protein • Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
Size:
10 μg100 μg
CAT.: P1HI0151S
Delivery: 2-3 weeks
Price: $ 660
OverviewKeyTec® GSK3β, N-GST recombinant protein with N-terminal GST tag was purified by GST affinity chromatography. The GSK3β protein showed high activity in ADP-Glo assay.
AA Sequences：Uniprot: P49841-1, M1-T420(end)
Tag： N-terminal GST tag
Molecular Weight： 72.6 kDa
Species：Human
Expression Host：Sf9
Protein Concentration：0.49 mg/mL by OD280
Purity：>70% by SDS-PAGE
Form：Liquid
Formulation：20 mM Hepes, 300 mM NaCl, 1 mM DTT, 10% glycerol, 0.05% Brij35, pH7.5
Performance
The GSK3β activity was detected using ADP-Glo technology. The reaction was performed by incubating the GSK3β protein, ATP and substrate at 25 ℃ for 60 min, then reading RLU by Microplate Reader with Luminescence.
Protocols
COA_P1HI0151_K130727012_EN.pdf
ComponentsCAT.
Description
Size
P1HI0151S
KeyTec® GSK3β, N-GST
10 μg
NoticesCertificate of Analysis
Limitations
Storage Conditions
For research use only
-80 ℃
Related ProductsOverviewKeyTec® GSK3β, N-GST recombinant protein with N-terminal GST tag was purified by GST affinity chromatography. The GSK3β protein showed high activity in ADP-Glo assay.
AA Sequences：Uniprot: P49841-1, M1-T420(end)
Tag： N-terminal GST tag
Molecular Weight： 72.6 kDa
Species：Human
Expression Host：Sf9
Protein Concentration：0.49 mg/mL by OD280
Purity：>70% by SDS-PAGE
Form：Liquid
Formulation：20 mM Hepes, 300 mM NaCl, 1 mM DTT, 10% glycerol, 0.05% Brij35, pH7.5
PerformanceThe GSK3β activity was detected using ADP-Glo technology. The reaction was performed by incubating the GSK3β protein, ATP and substrate at 25 ℃ for 60 min, then reading RLU by Microplate Reader with Luminescence.
ProtocolsCOA_P1HI0151_K130727012_EN.pdf
ComponentsCAT.
Description
Size
P1HI0151S
KeyTec® GSK3β, N-GST
10 μg
NoticesCertificate of Analysis
Limitations
Storage Conditions
For research use only
-80 ℃
Related Products沪ICP备2021012748号
WeChat
Sales Assistant:
Technical-Support:
WeChat Official Account:
Contact Us
Technical Support Email: technical-support@vkeybio.com
BD Email: business@vkeybio.com
Tel:
021-5835-6939
Cooperation