KeyTec® INSR, N-GST

    Used for Developing INSR Biochemical Activity Assay or Binding Assay Models

    • Strict quality control: Each batch comes with a rigorous QC report • High activity: Each batch is activity-verified, providing high-quality protein • Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
Size: 10 μg100 μg
Price: $ 660
CAT.: P1HI0065S
Delivery: 2-3 weeks
KeyTec® INSR, N-GST

    Used for Developing INSR Biochemical Activity Assay or Binding Assay Models

    • Strict quality control: Each batch comes with a rigorous QC report • High activity: Each batch is activity-verified, providing high-quality protein • Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
Size: 10 μg100 μg
CAT.: P1HI0065S
Delivery: 2-3 weeks
Price: $ 660
Overview
KeyTec® INSR, N-GST recombinant protein with N-terminal GST tag was purified by GST affinity and followed by SEC chromatography. The SEC-HPLC result showed that the INSR protein was dimer and polymer in the solution. The INSR protein showed high activity in TR-FRET assay. AA Sequences: Uniprot: P06213, V1005-K1310 Tag: N-terminal GST tag Molecular Weight: 60.5 kDa Species: Human Expression Host: Sf9 Protein Concentration: 1.65 mg/mL by OD280 Purity:> 95% by SDS-PAGE Form: Liquid Formulation: 20 mM Tris, 300 mM NaCl, 1 mM DTT, 10% glycerol, pH 7.5
Performance
The INSR activity was detected using TR-FRET technology. The reaction was performed by incubating the INSR protein, ATP and substrate at 25 ℃ for 60 min, adding the TR-FRET reagents at 25 ℃ for 60 min, then reading ratio 665/620 by Microplate Reader with TR-FRET.
Protocols
COA_P1HI0065_K130210013_EN.pdf
Components
CAT.
Description
Size
P1HI0065S
KeyTec® INSR, N-GST
10 μg
Notices
Certificate of Analysis
Limitations
Storage Conditions
For research use only

-80 ℃

Related Products
Overview
KeyTec® INSR, N-GST recombinant protein with N-terminal GST tag was purified by GST affinity and followed by SEC chromatography. The SEC-HPLC result showed that the INSR protein was dimer and polymer in the solution. The INSR protein showed high activity in TR-FRET assay. AA Sequences: Uniprot: P06213, V1005-K1310 Tag: N-terminal GST tag Molecular Weight: 60.5 kDa Species: Human Expression Host: Sf9 Protein Concentration: 1.65 mg/mL by OD280 Purity:> 95% by SDS-PAGE Form: Liquid Formulation: 20 mM Tris, 300 mM NaCl, 1 mM DTT, 10% glycerol, pH 7.5
Performance
The INSR activity was detected using TR-FRET technology. The reaction was performed by incubating the INSR protein, ATP and substrate at 25 ℃ for 60 min, adding the TR-FRET reagents at 25 ℃ for 60 min, then reading ratio 665/620 by Microplate Reader with TR-FRET.
Protocols
COA_P1HI0065_K130210013_EN.pdf
Components
CAT.
Description
Size
P1HI0065S
KeyTec® INSR, N-GST
10 μg
Notices
Certificate of Analysis
Limitations
Storage Conditions
For research use only

-80 ℃

Related Products

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