KeyTec® IRAK4, N-GST; C-StrepII
Used for Developing IRAK4 Biochemical Activity Assay or Binding Assay Models
• Strict quality control: Each batch comes with a rigorous QC report • High activity: Each batch is activity-verified, providing high-quality protein • Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
Size:
10 μg100 μg
Price: $ 660
CAT.: P1HI0066S
Delivery: 2-3 weeks
KeyTec® IRAK4, N-GST; C-StrepII
Used for Developing IRAK4 Biochemical Activity Assay or Binding Assay Models
• Strict quality control: Each batch comes with a rigorous QC report • High activity: Each batch is activity-verified, providing high-quality protein • Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
Size:
10 μg100 μg
CAT.: P1HI0066S
Delivery: 2-3 weeks
Price: $ 660
OverviewKeyTec® IRAK4, N-GST; C-StrepII recombinant protein with N-terminal GST tag and C-terminal StrepII tag was purified by GST affinity chromatography. The IRAK4 protein showed high activity in ADP-Glo assay.
AA Sequences： Uniprot: Q9NWZ3-1, M1-S460(end)
Tag： N-terminal GST-tag, C-terminal -StrepII
Molecular Weight： 78.5 kDa
Species： Human
Expression Host： Sf9
Protein Concentration： 0.3 mg/mL by OD280
Purity：> 95% by SDS-PAGE
Form： Liquid
Formulation： 50 mM Tris, 150 mM NaCl, 20 mM GSH, 1 mM DTT, 0.1 mM PMSF, 10% Glycerol, pH7.5
Performance
The IRAK4 activity was detected using ADP-Glo technology. The reaction was performed by incubating the IRAK4 protein, ATP and substrate at 25 ℃ for 60 min, then reading RLU by Microplate Reader with Luminescence.
Protocols
COA_P1HI0066_K130627011_EN.pdf
ComponentsCAT.
Description
Size
P1HI0066S
KeyTec® IRAK4, N-GST; C-StrepII
10 μg
NoticesCertificate of Analysis
Limitations
Storage Conditions
For research use only
-80 ℃
Related ProductsOverviewKeyTec® IRAK4, N-GST; C-StrepII recombinant protein with N-terminal GST tag and C-terminal StrepII tag was purified by GST affinity chromatography. The IRAK4 protein showed high activity in ADP-Glo assay.
AA Sequences： Uniprot: Q9NWZ3-1, M1-S460(end)
Tag： N-terminal GST-tag, C-terminal -StrepII
Molecular Weight： 78.5 kDa
Species： Human
Expression Host： Sf9
Protein Concentration： 0.3 mg/mL by OD280
Purity：> 95% by SDS-PAGE
Form： Liquid
Formulation： 50 mM Tris, 150 mM NaCl, 20 mM GSH, 1 mM DTT, 0.1 mM PMSF, 10% Glycerol, pH7.5
PerformanceThe IRAK4 activity was detected using ADP-Glo technology. The reaction was performed by incubating the IRAK4 protein, ATP and substrate at 25 ℃ for 60 min, then reading RLU by Microplate Reader with Luminescence.
ProtocolsCOA_P1HI0066_K130627011_EN.pdf
ComponentsCAT.
Description
Size
P1HI0066S
KeyTec® IRAK4, N-GST; C-StrepII
10 μg
NoticesCertificate of Analysis
Limitations
Storage Conditions
For research use only
-80 ℃
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