KeyTec® JAK1(JH1), N-GST
Used for Developing JAK1(JH1) Biochemical Activity Assay or Binding Assay Models
• Strict quality control: Each batch comes with a rigorous QC report • High activity: Each batch is activity-verified, providing high-quality protein • Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
Size:
10 μg100 μg
Price: $ 660
CAT.: P1HI0067S
Delivery: 2-3 weeks
KeyTec® JAK1(JH1), N-GST
Used for Developing JAK1(JH1) Biochemical Activity Assay or Binding Assay Models
• Strict quality control: Each batch comes with a rigorous QC report • High activity: Each batch is activity-verified, providing high-quality protein • Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
Size:
10 μg100 μg
CAT.: P1HI0067S
Delivery: 2-3 weeks
Price: $ 660
OverviewKeyTec® JAK1(JH1), N-GST recombinant protein with N-terminal GST tag + TEV cleavage site was purified by GST affinity and followed by SEC chromatography. The SEC-HPLC result showed that the JAK1(JH1) protein was polymer in the solution. The JAK1(JH1) protein showed high activity in ADP-Glo assay.
AA Sequences： Uniprot: P23458, D866-K1154(end)
Tag： N-terminal GST-tag
Molecular Weight： 59.8 kDa
Species： Human
Expression Host： Sf9
Protein Concentration： 0.26 mg/mL by OD280
Purity：> 75% by SDS-PAGE
Form： Liquid
Formulation： 20 mM Hepes, 300 mM NaCl, 20 mM Reduced Glu, 10% glycerol, pH 7.5
Performance
The JAK1(JH1) activity was detected using ADP-Glo technology. The reaction was performed by incubating the JAK1(JH1) protein, ATP and substrate at 25 ℃ for 60 min, then reading RLU by Microplate Reader with Luminescence.
Protocols
COA_P1HI0067_G130327011_EN.pdf
ComponentsCAT.
Description
Size
P1HI0067S
KeyTec® JAK1(JH1), N-GST
10 μg
NoticesCertificate of Analysis
Limitations
Storage Conditions
For research use only
-80 ℃
Related ProductsOverviewKeyTec® JAK1(JH1), N-GST recombinant protein with N-terminal GST tag + TEV cleavage site was purified by GST affinity and followed by SEC chromatography. The SEC-HPLC result showed that the JAK1(JH1) protein was polymer in the solution. The JAK1(JH1) protein showed high activity in ADP-Glo assay.
AA Sequences： Uniprot: P23458, D866-K1154(end)
Tag： N-terminal GST-tag
Molecular Weight： 59.8 kDa
Species： Human
Expression Host： Sf9
Protein Concentration： 0.26 mg/mL by OD280
Purity：> 75% by SDS-PAGE
Form： Liquid
Formulation： 20 mM Hepes, 300 mM NaCl, 20 mM Reduced Glu, 10% glycerol, pH 7.5
PerformanceThe JAK1(JH1) activity was detected using ADP-Glo technology. The reaction was performed by incubating the JAK1(JH1) protein, ATP and substrate at 25 ℃ for 60 min, then reading RLU by Microplate Reader with Luminescence.
ProtocolsCOA_P1HI0067_G130327011_EN.pdf
ComponentsCAT.
Description
Size
P1HI0067S
KeyTec® JAK1(JH1), N-GST
10 μg
NoticesCertificate of Analysis
Limitations
Storage Conditions
For research use only
-80 ℃
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