KeyTec® JAK3(JH1), N-His
Used for Developing JAK3(JH1) Biochemical Activity Assay or Binding Assay Models
• Strict quality control: Each batch comes with a rigorous QC report • High activity: Each batch is activity-verified, providing high-quality protein • Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
Size:
10 μg100 μg
Price: $ 660
CAT.: P1HI0068S
Delivery: 2-3 weeks
KeyTec® JAK3(JH1), N-His
Used for Developing JAK3(JH1) Biochemical Activity Assay or Binding Assay Models
• Strict quality control: Each batch comes with a rigorous QC report • High activity: Each batch is activity-verified, providing high-quality protein • Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
Size:
10 μg100 μg
CAT.: P1HI0068S
Delivery: 2-3 weeks
Price: $ 660
OverviewKeyTec® JAK3(JH1), N-His recombinant protein with N-terminal 8xHis tag was purified by Ni-NTA affinity chromatography. The JAK3(JH1) protein showed high activity in ADP-Glo assay.
AA Sequences：Uniprot: P52333-1, A795-S1124 (end)
Tag： N-terminal 8x His tag
Molecular Weight： 38.8 kDa
Species：Human
Expression Host：Sf9
Protein Concentration：0.51 mg/mL by OD280
Purity：>50% by SDS-PAGE
Form：Liquid
Formulation：20 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH8.0
Performance
The JAK3(JH1) activity was detected using ADP-Glo technology. The reaction was performed by incubating the JAK3(JH1) protein, ATP and substrate at 25 ℃ for 60 min, then reading RLU by Microplate Reader with Luminescence.
Protocols
COA_P1HI0068_F131211001_EN.pdf
ComponentsCAT.
Description
Size
P1HI0068S
KeyTec® JAK3(JH1), N-His
10 μg
NoticesCertificate of Analysis
Limitations
Storage Conditions
For research use only
-80 ℃
Related ProductsOverviewKeyTec® JAK3(JH1), N-His recombinant protein with N-terminal 8xHis tag was purified by Ni-NTA affinity chromatography. The JAK3(JH1) protein showed high activity in ADP-Glo assay.
AA Sequences：Uniprot: P52333-1, A795-S1124 (end)
Tag： N-terminal 8x His tag
Molecular Weight： 38.8 kDa
Species：Human
Expression Host：Sf9
Protein Concentration：0.51 mg/mL by OD280
Purity：>50% by SDS-PAGE
Form：Liquid
Formulation：20 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH8.0
PerformanceThe JAK3(JH1) activity was detected using ADP-Glo technology. The reaction was performed by incubating the JAK3(JH1) protein, ATP and substrate at 25 ℃ for 60 min, then reading RLU by Microplate Reader with Luminescence.
ProtocolsCOA_P1HI0068_F131211001_EN.pdf
ComponentsCAT.
Description
Size
P1HI0068S
KeyTec® JAK3(JH1), N-His
10 μg
NoticesCertificate of Analysis
Limitations
Storage Conditions
For research use only
-80 ℃
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