KeyTec® KAT1(HAT1), N-His-Flag

Used for Developing KAT1(HAT1) Biochemical Activity Assay or Binding Assay Models
•  Strict quality control: Each batch comes with a rigorous QC report
•  High activity: Each batch is activity-verified, providing high-quality protein
•  Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Size: 10 μg100 μg

Price: $ 456

CAT.: P2HI0019S

Delivery: 2-3 weeks

KeyTec® KAT1(HAT1), N-His-Flag

Used for Developing KAT1(HAT1) Biochemical Activity Assay or Binding Assay Models

• Strict quality control: Each batch comes with a rigorous QC report • High activity: Each batch is activity-verified, providing high-quality protein • Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Size: 10 μg100 μg

CAT.: P2HI0019S

Delivery: 2-3 weeks

Price: $ 456

Overview

KeyTec® KAT1(HAT1), N-HIS-FLAG recombinant protein with N-terminal 8x His tag and Flag tag was purified by Ni-NTA affinity and followed by SEC chromatography. The SEC-HPLC result showed that the KAT1 protein was monomer and dimer in the solution. The KAT1(HAT1) protein showed high activity in TR-FRET assay.
AA Sequences：Uniprot: O14929-1; M1-E419(end)
Tag：	 N-terminal 8x His and Flag tag
Molecular Weight：	 53.1 kDa
Species： Human
Expression Host： Sf9
Protein Concentration： 0.54 mg/mL by OD280
Purity：>95% by SDS-PAGE
Form： Liquid
Formulation： 20 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5

Performance

The KAT1(HAT1) activity was detected using TR-FRET technology. The reaction was performed by incubating the KAT1 protein and substrate at 25℃ for 90 min, adding the TR-FRET reagents at 25 ℃ for 60 min, then reading ratio 665/620 by Microplate Reader with TR-FRET. 

Protocols

COA_P2HI0019_G130308012_EN.pdf

Components

CAT.

Description

Size

P2HI0019S

KeyTec® KAT1(HAT1), N-His-Flag

10 μg

Notices

Certificate of Analysis

Limitations

Storage Conditions

For research use only

-80 ℃

Related Products

Overview

KeyTec® KAT1(HAT1), N-HIS-FLAG recombinant protein with N-terminal 8x His tag and Flag tag was purified by Ni-NTA affinity and followed by SEC chromatography. The SEC-HPLC result showed that the KAT1 protein was monomer and dimer in the solution. The KAT1(HAT1) protein showed high activity in TR-FRET assay.
AA Sequences：Uniprot: O14929-1; M1-E419(end)
Tag：	 N-terminal 8x His and Flag tag
Molecular Weight：	 53.1 kDa
Species： Human
Expression Host： Sf9
Protein Concentration： 0.54 mg/mL by OD280
Purity：>95% by SDS-PAGE
Form： Liquid
Formulation： 20 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5

Performance

The KAT1(HAT1) activity was detected using TR-FRET technology. The reaction was performed by incubating the KAT1 protein and substrate at 25℃ for 90 min, adding the TR-FRET reagents at 25 ℃ for 60 min, then reading ratio 665/620 by Microplate Reader with TR-FRET. 

Protocols

COA_P2HI0019_G130308012_EN.pdf

Components

CAT.

Description

Size

P2HI0019S

KeyTec® KAT1(HAT1), N-His-Flag

10 μg

Notices

Certificate of Analysis

Limitations