KeyTec® PARG, C-His M1-T976(end)

Used for Developing PARG Biochemical Activity Assay or Binding Assay Models
•  Strict quality control: Each batch comes with a rigorous QC report
•  High activity: Each batch is activity-verified, providing high-quality protein
•  Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Size: 10 μg100 μg

Price: $ 834

CAT.: P4HE0010S

Delivery: 2-3 weeks

KeyTec® PARG, C-His M1-T976(end)

Used for Developing PARG Biochemical Activity Assay or Binding Assay Models

• Strict quality control: Each batch comes with a rigorous QC report • High activity: Each batch is activity-verified, providing high-quality protein • Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Size: 10 μg100 μg

CAT.: P4HE0010S

Delivery: 2-3 weeks

Price: $ 834

Overview

KeyTec® PARG, C-His M1-T976(end) recombinant protein with N-terminal 8x His tag was purified by Ni-NTA affinity. The PARG protein showed high activity in TR-FRET assay.
AA Sequences：Uniprot: Q86W56-1, M1-T976(end)
Tag：	 C-terminal 8x His tag
Molecular Weight：	 112.3 kDa
Species： Human
Expression Host： E.coli
Protein Concentration： 0.41 mg/mL by OD280
Purity：>85% by SDS-PAGE
Form： Liquid
Formulation： 20 mM Tris, 200 mM NaCl, 1 mM DTT, 5% Glycerol, pH7.5

Performance

The PARG activity was detected using TR-FRET technology. The reaction was performed by incubating the PARG protein, substrate and the TR-FRET reagents at 25 ℃ for 60 min, then reading ratio 665/620 by Microplate Reader with TR-FRET. 

Protocols

COA_P4HE0010_120401_EN.pdf

Components

CAT.

Description

Size

P4HE0010S

KeyTec® PARG, C-His M1-T976(end)

10 μg

Notices

Certificate of Analysis

Limitations

Storage Conditions

For research use only

-80 ℃

Related Products

Overview

KeyTec® PARG, C-His M1-T976(end) recombinant protein with N-terminal 8x His tag was purified by Ni-NTA affinity. The PARG protein showed high activity in TR-FRET assay.
AA Sequences：Uniprot: Q86W56-1, M1-T976(end)
Tag：	 C-terminal 8x His tag
Molecular Weight：	 112.3 kDa
Species： Human
Expression Host： E.coli
Protein Concentration： 0.41 mg/mL by OD280
Purity：>85% by SDS-PAGE
Form： Liquid
Formulation： 20 mM Tris, 200 mM NaCl, 1 mM DTT, 5% Glycerol, pH7.5

Performance

The PARG activity was detected using TR-FRET technology. The reaction was performed by incubating the PARG protein, substrate and the TR-FRET reagents at 25 ℃ for 60 min, then reading ratio 665/620 by Microplate Reader with TR-FRET. 

Protocols

COA_P4HE0010_120401_EN.pdf

Components

CAT.

Description

Size

P4HE0010S

KeyTec® PARG, C-His M1-T976(end)

10 μg

Notices

Certificate of Analysis

Limitations