KeyTec® PARG, N-His
Used for Developing PARG Biochemical Activity Assay or Binding Assay Models
• Strict quality control: Each batch comes with a rigorous QC report • High activity: Each batch is activity-verified, providing high-quality protein • Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
Size:
10 μg100 μg
Price: $ 834
CAT.: P4HE0011S
Delivery: 2-3 weeks
KeyTec® PARG, N-His
Used for Developing PARG Biochemical Activity Assay or Binding Assay Models
• Strict quality control: Each batch comes with a rigorous QC report • High activity: Each batch is activity-verified, providing high-quality protein • Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
Size:
10 μg100 μg
CAT.: P4HE0011S
Delivery: 2-3 weeks
Price: $ 834
OverviewKeyTec® PARG, N-His recombinant protein with N-terminal 6x His tag was purified by Ni-NTA affinity. The PARG protein showed high activity in TR-FRET assay.
AA Sequences：Uniprot: Q86W56-1, S448-T976
Tag： N-terminal 6x His tag
Molecular Weight： 63.2 kDa
Species： Human
Expression Host： E.coli
Protein Concentration： 1.32 mg/mL by OD280
Purity：>70% by SDS-PAGE
Form： Liquid
Formulation： 20 mM Tris, 150 mM NaCl, 1 mM DTT, 10% Glycerol, pH7.5
Performance
The PARG activity was detected using TR-FRET technology. The reaction was performed by incubating the PARG protein, substrate and the TR-FRET reagents at 25 ℃ for 60 min, then reading ratio 665/620 by Microplate Reader with TR-FRET.
Protocols
COA_P4HE0011_D121027011_EN.pdf
ComponentsCAT.
Description
Size
P4HE0011S
KeyTec® PARG, N-His
10 μg
NoticesCertificate of Analysis
Limitations
Storage Conditions
For research use only
-80 ℃
Related ProductsOverviewKeyTec® PARG, N-His recombinant protein with N-terminal 6x His tag was purified by Ni-NTA affinity. The PARG protein showed high activity in TR-FRET assay.
AA Sequences：Uniprot: Q86W56-1, S448-T976
Tag： N-terminal 6x His tag
Molecular Weight： 63.2 kDa
Species： Human
Expression Host： E.coli
Protein Concentration： 1.32 mg/mL by OD280
Purity：>70% by SDS-PAGE
Form： Liquid
Formulation： 20 mM Tris, 150 mM NaCl, 1 mM DTT, 10% Glycerol, pH7.5
PerformanceThe PARG activity was detected using TR-FRET technology. The reaction was performed by incubating the PARG protein, substrate and the TR-FRET reagents at 25 ℃ for 60 min, then reading ratio 665/620 by Microplate Reader with TR-FRET.
ProtocolsCOA_P4HE0011_D121027011_EN.pdf
ComponentsCAT.
Description
Size
P4HE0011S
KeyTec® PARG, N-His
10 μg
NoticesCertificate of Analysis
Limitations
Storage Conditions
For research use only
-80 ℃
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