KeyTec® PLK3, N-GST
Used for Developing PLK3 Biochemical Activity Assay or Binding Assay Models
• Strict quality control: Each batch comes with a rigorous QC report • High activity: Each batch is activity-verified, providing high-quality protein • Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
Size:
10 μg100 μg
Price: $ 660
CAT.: P1HI0080S
Delivery: 2-3 weeks
KeyTec® PLK3, N-GST
Used for Developing PLK3 Biochemical Activity Assay or Binding Assay Models
• Strict quality control: Each batch comes with a rigorous QC report • High activity: Each batch is activity-verified, providing high-quality protein • Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
Size:
10 μg100 μg
CAT.: P1HI0080S
Delivery: 2-3 weeks
Price: $ 660
OverviewKeyTec® PLK3, N-GST recombinant protein with N-terminal GST tag + TEV cleavage site was purified by GST affinity chromatography. The PLK3 protein showed high activity in ADP-Glo assay.
AA Sequences： Uniprot: Q9H4B4, S58-A340
Tag： N-terminal GST tag
Molecular Weight： 58.9 kDa
Species： Human
Expression Host： Sf9
Protein Concentration： 0.50 mg/mL by OD280
Purity：> 95% by SDS-PAGE
Form： Liquid
Formulation： 20 mM Hepes, 300 mM NaCl, 1 mM DTT, 10% Glycerol, 0.05% Brij-35, pH7.0
Performance
The PLK3 activity was detected using ADP-Glo technology. The reaction was performed by incubating the PLK3 protein, ATP and substrate at 25 ℃ for 60 min, then reading RLU by Microplate Reader with Luminescence.
Protocols
COA_P1HI0080_K130509011_EN.pdf
ComponentsCAT.
Description
Size
P1HI0080S
KeyTec® PLK3, N-GST
10 μg
NoticesCertificate of Analysis
Limitations
Storage Conditions
For research use only
-80 ℃
Related ProductsOverviewKeyTec® PLK3, N-GST recombinant protein with N-terminal GST tag + TEV cleavage site was purified by GST affinity chromatography. The PLK3 protein showed high activity in ADP-Glo assay.
AA Sequences： Uniprot: Q9H4B4, S58-A340
Tag： N-terminal GST tag
Molecular Weight： 58.9 kDa
Species： Human
Expression Host： Sf9
Protein Concentration： 0.50 mg/mL by OD280
Purity：> 95% by SDS-PAGE
Form： Liquid
Formulation： 20 mM Hepes, 300 mM NaCl, 1 mM DTT, 10% Glycerol, 0.05% Brij-35, pH7.0
PerformanceThe PLK3 activity was detected using ADP-Glo technology. The reaction was performed by incubating the PLK3 protein, ATP and substrate at 25 ℃ for 60 min, then reading RLU by Microplate Reader with Luminescence.
ProtocolsCOA_P1HI0080_K130509011_EN.pdf
ComponentsCAT.
Description
Size
P1HI0080S
KeyTec® PLK3, N-GST
10 μg
NoticesCertificate of Analysis
Limitations
Storage Conditions
For research use only
-80 ℃
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