KeyTec® RET, N-GST; C-His

Used for Developing RET Biochemical Activity Assay or Binding Assay Models
•  Strict quality control: Each batch comes with a rigorous QC report
•  High activity: Each batch is activity-verified, providing high-quality protein
•  Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Size: 10 μg100 μg

Price: $ 660

CAT.: P1HI0082S

Delivery: 2-3 weeks

KeyTec® RET, N-GST; C-His

Used for Developing RET Biochemical Activity Assay or Binding Assay Models

• Strict quality control: Each batch comes with a rigorous QC report • High activity: Each batch is activity-verified, providing high-quality protein • Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Size: 10 μg100 μg

CAT.: P1HI0082S

Delivery: 2-3 weeks

Price: $ 660

Overview

KeyTec® RET, N-GST; C-His recombinant protein with N-terminal GST tag and C-terminal 8xHis tag was purified by GST affinity chromatography. The RET protein showed high activity in TR-FRET assay.
AA Sequences：	Uniprot: P07949, H658-S1114
Tag：		N-terminal GST-tag; C-terminal 8xHis tag
Molecular Weight：		78.6 kDa
Species：	Human
Expression Host：	Sf9
Protein Concentration：	0.38 mg/mL by OD280
Purity：> 70% by SDS-PAGE
Form：	Liquid
Formulation：	50 mM Tris, 150 mM NaCl, 20 mM GSH, 1 mM DTT, 0.1 mM PMSF, 10% Glycerol, 0.05% Brij-35, pH 7.5

Performance

The RET activity was detected using TR-FRET technology. The reaction was performed by incubating the RET protein, ATP and substrate at 25 ℃ for 60 min, adding the TR-FRET reagents at 25 ℃ for 60 min, then reading ratio 665/620 by Microplate Reader with TR-FRET.

Protocols

COA_P1HI0082_K130625011_EN.pdf

Components

CAT.

Description

Size

P1HI0082S

KeyTec® RET, N-GST; C-His

10 μg

Notices

Certificate of Analysis

Limitations

Storage Conditions

For research use only

-80 ℃

Related Products

Overview

KeyTec® RET, N-GST; C-His recombinant protein with N-terminal GST tag and C-terminal 8xHis tag was purified by GST affinity chromatography. The RET protein showed high activity in TR-FRET assay.
AA Sequences：	Uniprot: P07949, H658-S1114
Tag：		N-terminal GST-tag; C-terminal 8xHis tag
Molecular Weight：		78.6 kDa
Species：	Human
Expression Host：	Sf9
Protein Concentration：	0.38 mg/mL by OD280
Purity：> 70% by SDS-PAGE
Form：	Liquid
Formulation：	50 mM Tris, 150 mM NaCl, 20 mM GSH, 1 mM DTT, 0.1 mM PMSF, 10% Glycerol, 0.05% Brij-35, pH 7.5

Performance

The RET activity was detected using TR-FRET technology. The reaction was performed by incubating the RET protein, ATP and substrate at 25 ℃ for 60 min, adding the TR-FRET reagents at 25 ℃ for 60 min, then reading ratio 665/620 by Microplate Reader with TR-FRET.

Protocols

COA_P1HI0082_K130625011_EN.pdf

Components

CAT.

Description

Size

P1HI0082S

KeyTec® RET, N-GST; C-His

10 μg

Notices

Certificate of Analysis

Limitations