KeyTec® TR-FLISA® Enhancement Solution

KeyTec® TR-FLISA® Enhancement Solution is an acid solution containing chelating and detergent for quantitative determination of Eu3+/Sm3+ in a Time-Resolved Fluorescence Linked Immunosorbent Assay (TR-FLISA). Eu3+/Sm3+ labeling reagents are used to label macromolecules like proteins, peptides, and oligonucleotides, and the resulting markers are suitable for various types of analysis based on solid phase separations. After a series of manipulation steps, the remaining Eu3+/Sm3+-containing marker is immobilized onto a microtiter plate after the unbound portion is washed away. This solution is then added and can dissociate Eu3+/Sm3+ from the immobilized labeled antibody or protein within a few minutes, forming a homogeneous time-resolved fluorescent micellar chelate solution with extremely high brightness. Highly sensitive Eu3+/Sm3+ measurements can be performed using an instrument containing a time-resolved fluorescence module. Due to this solution dissociates Eu3+/Sm3+ into solution and produces an enhanced time-resolved fluorescence signal results in a low experimental background signal and a high upper measurement limit, thereby achieving a high signal-to-noise ratio and sensitivity.