KeyTec® TYK2(JH2), N-His-3C
Used for Developing TYK2(JH2) Biochemical Activity Assay or Binding Assay Models
• Strict quality control: Each batch comes with a rigorous QC report • High activity: Each batch is activity-verified, providing high-quality protein • Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
Size:
10 μg100 μg
Price: $ 660
CAT.: P1HI0098S
Delivery: 2-3 weeks
KeyTec® TYK2(JH2), N-His-3C
Used for Developing TYK2(JH2) Biochemical Activity Assay or Binding Assay Models
• Strict quality control: Each batch comes with a rigorous QC report • High activity: Each batch is activity-verified, providing high-quality protein • Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
Size:
10 μg100 μg
CAT.: P1HI0098S
Delivery: 2-3 weeks
Price: $ 660
OverviewKeyTec® TYK2(JH2), N-His-3C recombinant protein with N-terminal 8xHis tag and 3C cleavage site was purified by Ni-NTA affinity and followed by SEC chromatography. The SEC-HPLC result showed that the TYK2(JH2) protein was monomer in the solution. The TYK2(JH2) protein showed high activity in FP assay.
AA Sequences： Uniprot: P29597; G556-V877
Tag： N-terminal 8xHis tag
Molecular Weight： 38.2 kDa
Species： Human
Expression Host： Sf9
Protein Concentration： 0.29 mg/mL by OD280
Purity：>90% by SDS-PAGE
Form： Liquid
Formulation： 50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH 7.5
Performance
The TYK2(JH2) activity was detected using FP technology. The reaction was performed by incubating the TYK2(JH2) protein and
JH2 probe at 25 ℃ for 60 min, then reading fluorescence polarization by Microplate Reader.
Protocols
COA_P1HI0098_K130505012_EN.pdf
ComponentsCAT.
Description
Size
P1HI0098S
KeyTec® TYK2(JH2), N-His-3C
10 μg
NoticesCertificate of Analysis
Limitations
Storage Conditions
For research use only
-80 ℃
Related ProductsOverviewKeyTec® TYK2(JH2), N-His-3C recombinant protein with N-terminal 8xHis tag and 3C cleavage site was purified by Ni-NTA affinity and followed by SEC chromatography. The SEC-HPLC result showed that the TYK2(JH2) protein was monomer in the solution. The TYK2(JH2) protein showed high activity in FP assay.
AA Sequences： Uniprot: P29597; G556-V877
Tag： N-terminal 8xHis tag
Molecular Weight： 38.2 kDa
Species： Human
Expression Host： Sf9
Protein Concentration： 0.29 mg/mL by OD280
Purity：>90% by SDS-PAGE
Form： Liquid
Formulation： 50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH 7.5
PerformanceThe TYK2(JH2) activity was detected using FP technology. The reaction was performed by incubating the TYK2(JH2) protein and
JH2 probe at 25 ℃ for 60 min, then reading fluorescence polarization by Microplate Reader.
ProtocolsCOA_P1HI0098_K130505012_EN.pdf
ComponentsCAT.
Description
Size
P1HI0098S
KeyTec® TYK2(JH2), N-His-3C
10 μg
NoticesCertificate of Analysis
Limitations
Storage Conditions
For research use only
-80 ℃
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