KeyTec® BRAF, N-GST; C-His
Used for Developing BRAF Biochemical Activity Assay or Binding Assay Models
• Strict quality control: Each batch comes with a rigorous QC report • High activity: Each batch is activity-verified, providing high-quality protein • Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
Size:
10 μg100 μg
Price: $ 661
CAT.: P1HI0234S
Delivery: 2-3 weeks
KeyTec® BRAF, N-GST; C-His
Used for Developing BRAF Biochemical Activity Assay or Binding Assay Models
• Strict quality control: Each batch comes with a rigorous QC report • High activity: Each batch is activity-verified, providing high-quality protein • Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
Size:
10 μg100 μg
CAT.: P1HI0234S
Delivery: 2-3 weeks
Price: $ 661
OverviewKeyTec® BRAF, N-GST; C-His recombinant protein with N-terminal GST tag and C-terminal 6x His tag was purified by Ni-NTA affinity and followed by GST affinity chromatography. The BRAF protein showed high activity in ADP-Glo assay.
AA Sequences： Uniprot: P15056, E433-R726
Tag： N-terminal GST tag, C-terminal 6x His tag
Molecular Weight： 61.3 kDa
Species： Human
Expression Host： Sf9
Protein Concentration： 1.00 mg/mL by OD280
Purity： > 80% by SDS-PAGE
Form： Liquid
Formulation： 50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5
Performance
The BRAF activity was detected using ADP-Glo technology. The reaction was performed by incubating the BRAF protein, ATP and substrate at 25 ℃ for 60 min, then reading RLU by Microplate Reader with Luminescence.
Protocols
COA_P1HI0234_G140223021_EN.pdf
ComponentsCAT.
Description
Size
P1HI0234S
KeyTec® BRAF, N-GST; C-His
10 μg
NoticesCertificate of Analysis
Limitations
Storage Conditions
For research use only
-80 ℃
Related ProductsOverviewKeyTec® BRAF, N-GST; C-His recombinant protein with N-terminal GST tag and C-terminal 6x His tag was purified by Ni-NTA affinity and followed by GST affinity chromatography. The BRAF protein showed high activity in ADP-Glo assay.
AA Sequences： Uniprot: P15056, E433-R726
Tag： N-terminal GST tag, C-terminal 6x His tag
Molecular Weight： 61.3 kDa
Species： Human
Expression Host： Sf9
Protein Concentration： 1.00 mg/mL by OD280
Purity： > 80% by SDS-PAGE
Form： Liquid
Formulation： 50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5
PerformanceThe BRAF activity was detected using ADP-Glo technology. The reaction was performed by incubating the BRAF protein, ATP and substrate at 25 ℃ for 60 min, then reading RLU by Microplate Reader with Luminescence.
ProtocolsCOA_P1HI0234_G140223021_EN.pdf
ComponentsCAT.
Description
Size
P1HI0234S
KeyTec® BRAF, N-GST; C-His
10 μg
NoticesCertificate of Analysis
Limitations
Storage Conditions
For research use only
-80 ℃
Related Products沪ICP备2021012748号
WeChat
Sales Assistant:
Technical-Support:
WeChat Official Account:
Contact Us
Technical Support Email: technical-support@vkeybio.com
BD Email: business@vkeybio.com
Tel:
021-5835-6939
Cooperation