KeyTec® BTK[C481S], N-GST

Used for Developing BTK[C481R] Biochemical Activity Assay or Binding Assay Models
•  Strict quality control: Each batch comes with a rigorous QC report
•  High activity: Each batch is activity-verified, providing high-quality protein
•  Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Size: 10 μg100 μg

Price: $ 945

CAT.: P1HI0008S

Delivery: 2-3 weeks

KeyTec® BTK[C481S], N-GST

Used for Developing BTK[C481R] Biochemical Activity Assay or Binding Assay Models

• Strict quality control: Each batch comes with a rigorous QC report • High activity: Each batch is activity-verified, providing high-quality protein • Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Size: 10 μg100 μg

CAT.: P1HI0008S

Delivery: 2-3 weeks

Price: $ 945

Overview

KeyTec® BTK[C481S], N-GST recombinant protein with N-terminal GST tag was purified by GST affinity and followed by SEC chromatography. The SEC-HPLC result showed that the BTK[C481S] protein was dimer in the solution. The BTK[C481S] protein showed high activity in TR-FRET assay.
AA Sequences：Uniprot:  Q06187, A2-S659, C481S
Tag：N-terminal GST tag
Molecular Weight：101.8 kDa
Species：Human
Expression Host：Sf9
Protein Concentration：0.41 mg/mL by OD280
Purity：> 80% by SDS-PAGE
Form：Liquid
Formulation：50 mM Hepes, 150 mM NaCl, 20 mM Reduced Glu, 1 mM DTT, 10% glycerol, pH 7.5

Performance

The BTK[C481S] activity was detected using TR-FRET technology. The reaction was performed by incubating the BTK[C481S] protein, ATP and substrate at 25 ℃ for 60 min, adding the TR-FRET reagents at 25℃ for 60 min, then reading ratio 665/620 by Microplate Reader with TR-FRET.

Protocols

COA_P1HI0008_K130320011_EN.pdf

Components

CAT.

Description

Size

P1HI0008S

KeyTec® BTK[C481S], N-GST

10 μg

Notices

Certificate of Analysis

Limitations

Storage Conditions

For research use only

-80 ℃

Related Products

Overview

KeyTec® BTK[C481S], N-GST recombinant protein with N-terminal GST tag was purified by GST affinity and followed by SEC chromatography. The SEC-HPLC result showed that the BTK[C481S] protein was dimer in the solution. The BTK[C481S] protein showed high activity in TR-FRET assay.
AA Sequences：Uniprot:  Q06187, A2-S659, C481S
Tag：N-terminal GST tag
Molecular Weight：101.8 kDa
Species：Human
Expression Host：Sf9
Protein Concentration：0.41 mg/mL by OD280
Purity：> 80% by SDS-PAGE
Form：Liquid
Formulation：50 mM Hepes, 150 mM NaCl, 20 mM Reduced Glu, 1 mM DTT, 10% glycerol, pH 7.5

Performance

The BTK[C481S] activity was detected using TR-FRET technology. The reaction was performed by incubating the BTK[C481S] protein, ATP and substrate at 25 ℃ for 60 min, adding the TR-FRET reagents at 25℃ for 60 min, then reading ratio 665/620 by Microplate Reader with TR-FRET.

Protocols

COA_P1HI0008_K130320011_EN.pdf

Components

CAT.

Description

Size

P1HI0008S

KeyTec® BTK[C481S], N-GST

10 μg

Notices

Certificate of Analysis

Limitations