KeyTec® BTK[T474S], N-GST
Used for Developing BTK[T474S] Biochemical Activity Assay or Binding Assay Models
• Strict quality control: Each batch comes with a rigorous QC report • High activity: Each batch is activity-verified, providing high-quality protein • Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
Size:
10 μg100 μg
Price: $ 945
CAT.: P1HI0012S
Delivery: 2-3 weeks
KeyTec® BTK[T474S], N-GST
Used for Developing BTK[T474S] Biochemical Activity Assay or Binding Assay Models
• Strict quality control: Each batch comes with a rigorous QC report • High activity: Each batch is activity-verified, providing high-quality protein • Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
Size:
10 μg100 μg
CAT.: P1HI0012S
Delivery: 2-3 weeks
Price: $ 945
OverviewKeyTec® BTK[T474S], N-GST recombinant protein with N-terminal GST tag was purified by GST affinity chromatography. The BTK[T474S] protein showed high activity in TR-FRET assay.
AA Sequences：Uniprot: Q06187-1, A2-S659(end), T474S
Tag：N-terminal GST tag
Molecular Weight：101.9 kDa
Species：Human
Expression Host：Sf9
Protein Concentration：0.44 mg/mL by OD280
Purity：> 90% by SDS-PAGE
Form：Liquid
Formulation：20 mM Hepes, 300 mM NaCl, 1 mM DTT, 0.1 mM PMSF, 10% Glycerol, 0.05% Brij-35, pH7.5
Performance
The BTK[T474S] activity was detected using TR-FRET technology. The reaction was performed by incubating the BTK[T474S] protein, ATP and substrate at 25 ℃ for 60 min, adding the TR-FRET reagents at 25℃ for 60 min, then reading ratio 665/620 by Microplate Reader with TR-FRET.
Protocols
COA_P1HI0012_K130506011_EN.pdf
ComponentsCAT.
Description
Size
P1HI0012S
KeyTec® BTK[T474S], N-GST
10 μg
NoticesCertificate of Analysis
Limitations
Storage Conditions
For research use only
-80 ℃
Related ProductsOverviewKeyTec® BTK[T474S], N-GST recombinant protein with N-terminal GST tag was purified by GST affinity chromatography. The BTK[T474S] protein showed high activity in TR-FRET assay.
AA Sequences：Uniprot: Q06187-1, A2-S659(end), T474S
Tag：N-terminal GST tag
Molecular Weight：101.9 kDa
Species：Human
Expression Host：Sf9
Protein Concentration：0.44 mg/mL by OD280
Purity：> 90% by SDS-PAGE
Form：Liquid
Formulation：20 mM Hepes, 300 mM NaCl, 1 mM DTT, 0.1 mM PMSF, 10% Glycerol, 0.05% Brij-35, pH7.5
PerformanceThe BTK[T474S] activity was detected using TR-FRET technology. The reaction was performed by incubating the BTK[T474S] protein, ATP and substrate at 25 ℃ for 60 min, adding the TR-FRET reagents at 25℃ for 60 min, then reading ratio 665/620 by Microplate Reader with TR-FRET.
ProtocolsCOA_P1HI0012_K130506011_EN.pdf
ComponentsCAT.
Description
Size
P1HI0012S
KeyTec® BTK[T474S], N-GST
10 μg
NoticesCertificate of Analysis
Limitations
Storage Conditions
For research use only
-80 ℃
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