KeyTec® CDK1/CycA2, N-GST/N-GST;C-His
Used for Developing CDK1/CycA2 Biochemical Activity Assay or Binding Assay Models
• Strict quality control: Each batch comes with a rigorous QC report • High activity: Each batch is activity-verified, providing high-quality protein • Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
Size:
10 μg100 μg
Price: $ 660
CAT.: P1HI0105S
Delivery: 2-3 weeks
KeyTec® CDK1/CycA2, N-GST/N-GST;C-His
Used for Developing CDK1/CycA2 Biochemical Activity Assay or Binding Assay Models
• Strict quality control: Each batch comes with a rigorous QC report • High activity: Each batch is activity-verified, providing high-quality protein • Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
Size:
10 μg100 μg
CAT.: P1HI0105S
Delivery: 2-3 weeks
Price: $ 660
OverviewKeyTec® CDK1/CycA2, N-GST/N-GST;C-His recombinant protein was co-expressed recombinant human CDK1 with N-terminal GST tag, CycA2 protein with N-terminal GST tag and C- terminal 8x His tag. Recombinant human CDK1/CycA2 protein was purified by GST affinity and followed by SEC chromatography. The CDK1/CycA2 protein showed high activity in ADP-Glo assay.
AA Sequences： Uniprot: CDK1: P06493-1, M1-M297(end); CycA2: P20248, E174-L432(end)
Tag： CDK1: N-terminal GST tag; CycA2: N-terminal GST tag, C-terminal 8x His tag
Molecular Weight： CDK1: 60.7 kDa; CycA2: 56.8 kDa
Species： Human
Expression Host： Sf9
Protein Concentration： 0.99 mg/mL by OD280
Purity： >95% by SDS-PAGE
Form： Liquid
Formulation： 50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, 0.05% Brij35, pH7.5
Performance
The CDK1/CycA2 activity was detected using ADP-Glo technology. The reaction was performed by incubating the CDK1/CycA2 protein, ATP and substrate at 25 ℃ for 60 min, then reading RLU by Microplate Reader with Luminescence.
Protocols
COA_P1HI0105_K130801012_EN.pdf
ComponentsCAT.
Description
Size
P1HI0105S
KeyTec® CDK1/CycA2, N-GST/N-GST;C-His
10 μg
NoticesCertificate of Analysis
Limitations
Storage Conditions
For research use only
-80 ℃
Related ProductsOverviewKeyTec® CDK1/CycA2, N-GST/N-GST;C-His recombinant protein was co-expressed recombinant human CDK1 with N-terminal GST tag, CycA2 protein with N-terminal GST tag and C- terminal 8x His tag. Recombinant human CDK1/CycA2 protein was purified by GST affinity and followed by SEC chromatography. The CDK1/CycA2 protein showed high activity in ADP-Glo assay.
AA Sequences： Uniprot: CDK1: P06493-1, M1-M297(end); CycA2: P20248, E174-L432(end)
Tag： CDK1: N-terminal GST tag; CycA2: N-terminal GST tag, C-terminal 8x His tag
Molecular Weight： CDK1: 60.7 kDa; CycA2: 56.8 kDa
Species： Human
Expression Host： Sf9
Protein Concentration： 0.99 mg/mL by OD280
Purity： >95% by SDS-PAGE
Form： Liquid
Formulation： 50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, 0.05% Brij35, pH7.5
PerformanceThe CDK1/CycA2 activity was detected using ADP-Glo technology. The reaction was performed by incubating the CDK1/CycA2 protein, ATP and substrate at 25 ℃ for 60 min, then reading RLU by Microplate Reader with Luminescence.
ProtocolsCOA_P1HI0105_K130801012_EN.pdf
ComponentsCAT.
Description
Size
P1HI0105S
KeyTec® CDK1/CycA2, N-GST/N-GST;C-His
10 μg
NoticesCertificate of Analysis
Limitations
Storage Conditions
For research use only
-80 ℃
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