KeyTec® CDK13/Cyclin K, N-GST/N-FLAG; C-His
Used for Developing CDK13/Cyclin K Biochemical Activity Assay or Binding Assay Models
• Strict quality control: Each batch comes with a rigorous QC report • High activity: Each batch is activity-verified, providing high-quality protein • Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
Size:
10 μg100 μg
Price: $ 945
CAT.: P1HI0018S
Delivery: 2-3 weeks
KeyTec® CDK13/Cyclin K, N-GST/N-FLAG; C-His
Used for Developing CDK13/Cyclin K Biochemical Activity Assay or Binding Assay Models
• Strict quality control: Each batch comes with a rigorous QC report • High activity: Each batch is activity-verified, providing high-quality protein • Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
Size:
10 μg100 μg
CAT.: P1HI0018S
Delivery: 2-3 weeks
Price: $ 945
OverviewKeyTec® CDK13/Cyclin K, N-GST/ N-FLAG; C-His recombinant protein was co-expressed recombinant human CDK13 with N-terminal GST tag and Cyclin K protein with N-terminal GST tag and C-terminal 6xHis tag. The complex was purified by GST affinity and followed by SEC chromatography. The SEC-HPLC result showed that the CDK13/Cyclin K protein was polymer in the solution. The CDK13/Cyclin K protein showed high activity in ADP-Glo assay.
AA Sequences： Uniprot: CDK13: Q14004, Q673-S1054; Cyclin K: O75909, M1-S300
Tag： CDK13: N-terminal GST tag; Cyclin K: N-terminal-Flag, C-His
Molecular Weight： CDK13: 70.3 kDa; Cyclin K: 37.2 kDa
Species： Human
Expression Host： Sf9
Protein Concentration： 0.33 mg/mL by OD280
Purity：> 85% by SDS-PAGE
Form： Liquid
Formulation： 50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, 0.05% Brij35, pH 7.5
Performance
The CDK13/Cyclin K activity was detected using ADP-Glo technology. The reaction was performed by incubating the CDK13/Cyclin K protein, ATP and substrate at 25 ℃ for 60 min, then reading RLU by Microplate Reader with Luminescence.
Protocols
COA_P1HI0018_G130626011_EN.pdf
ComponentsCAT.
Description
Size
P1HI0018S
KeyTec® CDK13/Cyclin K, N-GST/N-FLAG; C-His
10 μg
NoticesCertificate of Analysis
Limitations
Storage Conditions
For research use only
-80 ℃
Related ProductsOverviewKeyTec® CDK13/Cyclin K, N-GST/ N-FLAG; C-His recombinant protein was co-expressed recombinant human CDK13 with N-terminal GST tag and Cyclin K protein with N-terminal GST tag and C-terminal 6xHis tag. The complex was purified by GST affinity and followed by SEC chromatography. The SEC-HPLC result showed that the CDK13/Cyclin K protein was polymer in the solution. The CDK13/Cyclin K protein showed high activity in ADP-Glo assay.
AA Sequences： Uniprot: CDK13: Q14004, Q673-S1054; Cyclin K: O75909, M1-S300
Tag： CDK13: N-terminal GST tag; Cyclin K: N-terminal-Flag, C-His
Molecular Weight： CDK13: 70.3 kDa; Cyclin K: 37.2 kDa
Species： Human
Expression Host： Sf9
Protein Concentration： 0.33 mg/mL by OD280
Purity：> 85% by SDS-PAGE
Form： Liquid
Formulation： 50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, 0.05% Brij35, pH 7.5
PerformanceThe CDK13/Cyclin K activity was detected using ADP-Glo technology. The reaction was performed by incubating the CDK13/Cyclin K protein, ATP and substrate at 25 ℃ for 60 min, then reading RLU by Microplate Reader with Luminescence.
ProtocolsCOA_P1HI0018_G130626011_EN.pdf
ComponentsCAT.
Description
Size
P1HI0018S
KeyTec® CDK13/Cyclin K, N-GST/N-FLAG; C-His
10 μg
NoticesCertificate of Analysis
Limitations
Storage Conditions
For research use only
-80 ℃
Related Products沪ICP备2021012748号
WeChat
Sales Assistant:
Technical-Support:
WeChat Official Account:
Contact Us
Technical Support Email: technical-support@vkeybio.com
BD Email: business@vkeybio.com
Tel:
021-5835-6939
Cooperation