KeyTec® CDK9/Cyclin K, N-GST/N-Flag;C-His
Used for Developing CDK9/Cyclin K Biochemical Activity Assay or Binding Assay Models
• Strict quality control: Each batch comes with a rigorous QC report • High activity: Each batch is activity-verified, providing high-quality protein • Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
Size:
10 μg100 μg
Price: $ 660
CAT.: P1HI0111S
Delivery: 2-3 weeks
KeyTec® CDK9/Cyclin K, N-GST/N-Flag;C-His
Used for Developing CDK9/Cyclin K Biochemical Activity Assay or Binding Assay Models
• Strict quality control: Each batch comes with a rigorous QC report • High activity: Each batch is activity-verified, providing high-quality protein • Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
Size:
10 μg100 μg
CAT.: P1HI0111S
Delivery: 2-3 weeks
Price: $ 660
OverviewKeyTec® CDK9/Cyclin K, N-GST/N-Flag;C-His recombinant protein was co-expressed recombinant human CDK9 with N-terminal GST tag, Cyclin K with N-terminal Flag tag and C-terminal 6xHis tag. The complex was purified by Ni-NTA affinity chromatography. The CDK9/Cyclin K protein showed high activity in ADP-Glo assay.
AA Sequences： Uniprot: CDK9:P50750-1, M1-F372(end); Cyclin K: O75909, M1-S300
Tag： CDK9:N-terminal GST tag; Cyclin K: N-terminal Flag tag, C-terminal 6x His tag
Molecular Weight： CDK9:68.6 kDa; Cyclin K: 37.2 kDa
Species： Human
Expression Host： Sf9
Protein Concentration： 0.36 mg/mL by OD280
Purity：> 95% by SDS-PAGE
Form： Liquid
Formulation： 50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, 0.05% Brij35, pH7.5
Performance
The CDK9/Cyclin K activity was detected using ADP-Glo technology. The reaction was performed by incubating the CDK9/Cyclin K protein, ATP and substrate at 25 ℃ for 60 min, then reading RLU by Microplate Reader with Luminescence.
Protocols
COA_P1HI0111_K130808011_EN.pdf
ComponentsCAT.
Description
Size
P1HI0111S
KeyTec® CDK9/Cyclin K, N-GST/N-Flag;C-His
10 μg
NoticesCertificate of Analysis
Limitations
Storage Conditions
For research use only
-80 ℃
Related ProductsOverviewKeyTec® CDK9/Cyclin K, N-GST/N-Flag;C-His recombinant protein was co-expressed recombinant human CDK9 with N-terminal GST tag, Cyclin K with N-terminal Flag tag and C-terminal 6xHis tag. The complex was purified by Ni-NTA affinity chromatography. The CDK9/Cyclin K protein showed high activity in ADP-Glo assay.
AA Sequences： Uniprot: CDK9:P50750-1, M1-F372(end); Cyclin K: O75909, M1-S300
Tag： CDK9:N-terminal GST tag; Cyclin K: N-terminal Flag tag, C-terminal 6x His tag
Molecular Weight： CDK9:68.6 kDa; Cyclin K: 37.2 kDa
Species： Human
Expression Host： Sf9
Protein Concentration： 0.36 mg/mL by OD280
Purity：> 95% by SDS-PAGE
Form： Liquid
Formulation： 50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, 0.05% Brij35, pH7.5
PerformanceThe CDK9/Cyclin K activity was detected using ADP-Glo technology. The reaction was performed by incubating the CDK9/Cyclin K protein, ATP and substrate at 25 ℃ for 60 min, then reading RLU by Microplate Reader with Luminescence.
ProtocolsCOA_P1HI0111_K130808011_EN.pdf
ComponentsCAT.
Description
Size
P1HI0111S
KeyTec® CDK9/Cyclin K, N-GST/N-Flag;C-His
10 μg
NoticesCertificate of Analysis
Limitations
Storage Conditions
For research use only
-80 ℃
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