KeyTec® CK2α2[C336S]/CK2β, N-GST/N-His

Used for Developing CK2α2[C336S]/CK2β Biochemical Activity Assay or Binding Assay Models
•  Strict quality control: Each batch comes with a rigorous QC report
•  High activity: Each batch is activity-verified, providing high-quality protein
•  Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Size: 10 μg100 μg

Price: $ 660

CAT.: P1HI0029S

Delivery: 2-3 weeks

KeyTec® CK2α2[C336S]/CK2β, N-GST/N-His

Used for Developing CK2α2[C336S]/CK2β Biochemical Activity Assay or Binding Assay Models

• Strict quality control: Each batch comes with a rigorous QC report • High activity: Each batch is activity-verified, providing high-quality protein • Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Size: 10 μg100 μg

CAT.: P1HI0029S

Delivery: 2-3 weeks

Price: $ 660

Overview

KeyTec® CK2α2[C336S]/CK2β, N-GST/N-His recombinant protein was co-expressed recombinant human CK2α2[C336S] with N-terminal GST tag and CK2β protein with N-terminal 6xHis tag. The complex was purified by Ni-NTA affinity chromatography. The CK2α2[C336S]/CK2β protein showed high activity in ADP-Glo assay.
AA Sequences：	Uniprot: CK2α2: P19784, M1-R350(end),C336S; CK2β: N0E4C7, M1-R215(end)
Tag：		CK2α2[C336S]: N-terminal GST tag; CK2β: N-terminal 6x His tag
Molecular Weight：		CK2α2[C336S]: 67.8 kDa; CK2β: 25.9 kDa
Species：	Human
Expression Host：	Sf9
Protein Concentration：	0.83 mg/mL by OD280
Purity：> 90% by SDS-PAGE
Form：	Liquid
Formulation：	50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, 0.05% Brij35, pH7.5

Performance

The CK2α2[C336S]/CK2β activity was detected using ADP-Glo technology. The reaction was performed by incubating the CK2α2[C336S]/CK2β protein, ATP and substrate at 25 ℃ for 60 min, then reading RLU by Microplate Reader with Luminescence.

Protocols

COA_P1HI0029_G130706011_EN.pdf

Components

CAT.

Description

Size

P1HI0029S

KeyTec® CK2α2[C336S]/CK2β, N-GST/N-His

10 μg

Notices

Certificate of Analysis

Limitations

Storage Conditions

For research use only

-80 ℃

Related Products

Overview

KeyTec® CK2α2[C336S]/CK2β, N-GST/N-His recombinant protein was co-expressed recombinant human CK2α2[C336S] with N-terminal GST tag and CK2β protein with N-terminal 6xHis tag. The complex was purified by Ni-NTA affinity chromatography. The CK2α2[C336S]/CK2β protein showed high activity in ADP-Glo assay.
AA Sequences：	Uniprot: CK2α2: P19784, M1-R350(end),C336S; CK2β: N0E4C7, M1-R215(end)
Tag：		CK2α2[C336S]: N-terminal GST tag; CK2β: N-terminal 6x His tag
Molecular Weight：		CK2α2[C336S]: 67.8 kDa; CK2β: 25.9 kDa
Species：	Human
Expression Host：	Sf9
Protein Concentration：	0.83 mg/mL by OD280
Purity：> 90% by SDS-PAGE
Form：	Liquid
Formulation：	50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, 0.05% Brij35, pH7.5

Performance

The CK2α2[C336S]/CK2β activity was detected using ADP-Glo technology. The reaction was performed by incubating the CK2α2[C336S]/CK2β protein, ATP and substrate at 25 ℃ for 60 min, then reading RLU by Microplate Reader with Luminescence.

Protocols

COA_P1HI0029_G130706011_EN.pdf

Components

CAT.

Description

Size

P1HI0029S

KeyTec® CK2α2[C336S]/CK2β, N-GST/N-His

10 μg

Notices

Certificate of Analysis

Limitations