KeyTec® D-LUCIFERIN POTASSIUM SALT is designed for imaging living Firefly luciferase reporter gene cells in vitro or in vivo. It is a highly efficient, stable and reliable substrate for Firefly luciferase, producing a sensitive and robust luminescence signal. Its excellent water and fat solubility properties allow it to easily cross cell membranes.
The detection principle is based on Luminescent technology. D-Luciferin-K reacts with Firefly luciferase in cells, generating a sensitive and robust luminescence signal. The process provides a highly sensitive, robust, and homogeneous assay for the imaging living Firefly luciferase reporter gene cells, whether in vitro or in vivo.