KeyTec® EPHA1, N-GST
Used for Developing EPHA1 Biochemical Activity Assay or Binding Assay Models
• Strict quality control: Each batch comes with a rigorous QC report • High activity: Each batch is activity-verified, providing high-quality protein • Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
Size:
10 μg100 μg
Price: $ 660
CAT.: P1HI0040S
Delivery: 2-3 weeks
KeyTec® EPHA1, N-GST
Used for Developing EPHA1 Biochemical Activity Assay or Binding Assay Models
• Strict quality control: Each batch comes with a rigorous QC report • High activity: Each batch is activity-verified, providing high-quality protein • Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
Size:
10 μg100 μg
CAT.: P1HI0040S
Delivery: 2-3 weeks
Price: $ 660
OverviewKeyTec® EPHA1, N-GST recombinant protein with N-terminal GST tag + TEV cleavage site was purified by GST affinity and followed by SEC chromatography. The SEC-HPLC result showed that the EPHA1 protein was dimer and polymer in the solution. The EPHA1 protein showed high activity in TR-FRET assay.
AA Sequences： Uniprot: P21709, T586-D976
Tag： N-terminal GST tag
Molecular Weight： 71.2 kDa
Species： Human
Expression Host： Sf9
Protein Concentration： 0.82 mg/mL by OD280
Purity：>80% by SDS-PAGE
Form： Liquid
Formulation： 50 mM Tris, 150 mM NaCl, 20mM Reduced Glu, 1mM DTT, 0.05% Brij-35, 10% glycerol, pH 8.0
Performance
The EPHA1 activity was detected using TR-FRET technology. The reaction was performed by incubating the EPHA1 protein, ATP and substrate at 25 ℃ for 50 min, adding the TR-FRET reagents at 25℃ for 60 min, then reading ratio 665/620 by Microplate Reader with TR-FRET.
Protocols
COA_P1HI0040_K130403011_EN.pdf
ComponentsCAT.
Description
Size
P1HI0040S
KeyTec® EPHA1, N-GST
10 μg
NoticesCertificate of Analysis
Limitations
Storage Conditions
For research use only
-80 ℃
Related ProductsOverviewKeyTec® EPHA1, N-GST recombinant protein with N-terminal GST tag + TEV cleavage site was purified by GST affinity and followed by SEC chromatography. The SEC-HPLC result showed that the EPHA1 protein was dimer and polymer in the solution. The EPHA1 protein showed high activity in TR-FRET assay.
AA Sequences： Uniprot: P21709, T586-D976
Tag： N-terminal GST tag
Molecular Weight： 71.2 kDa
Species： Human
Expression Host： Sf9
Protein Concentration： 0.82 mg/mL by OD280
Purity：>80% by SDS-PAGE
Form： Liquid
Formulation： 50 mM Tris, 150 mM NaCl, 20mM Reduced Glu, 1mM DTT, 0.05% Brij-35, 10% glycerol, pH 8.0
PerformanceThe EPHA1 activity was detected using TR-FRET technology. The reaction was performed by incubating the EPHA1 protein, ATP and substrate at 25 ℃ for 50 min, adding the TR-FRET reagents at 25℃ for 60 min, then reading ratio 665/620 by Microplate Reader with TR-FRET.
ProtocolsCOA_P1HI0040_K130403011_EN.pdf
ComponentsCAT.
Description
Size
P1HI0040S
KeyTec® EPHA1, N-GST
10 μg
NoticesCertificate of Analysis
Limitations
Storage Conditions
For research use only
-80 ℃
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