KeyTec® EPHA4, N-GST recombinant protein with N-terminal GST tag + TEV cleavage site was purified by GST affinity and followed by SEC chromatography. The SEC-HPLC result showed that the EPHA4 protein was trimer in the solution. The EPHA4 protein showed high activity in TR-FRET assay.
AA Sequences: Uniprot: P54764, E586-V986
Tag: N-terminal GST tag
Molecular Weight: 71.9 kDa
Species: Human
Expression Host: Sf9
Protein Concentration: 1.10 mg/mL by OD280
Purity:>90% by SDS-PAGE
Form: Liquid
Formulation: 50 mM Tris, 150 mM NaCl, 20mM Reduced Glu, 0.05% Brij-35, 1mM DTT, 10% glycerol, pH7.5