KeyTec® Enhanced Luciferase Detection Kit (no DTT)

Luminescent Assay for Detecting Firefly Luciferase in Reporter Gene Assays
•  Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
•  High Sensitivity: Wider linear range and robust luminescence signal and without DTT
•  The luminescence signal is not only high but also robust, with a half-life of approximately 1 h. It is suitable for most assay formats

Size: 10 mL2*50 mL2*250 mL

Price: $ 125

CAT.: A2000600N

Delivery: Available in Stock

KeyTec® Enhanced Luciferase Detection Kit (no DTT)

Luminescent Assay for Detecting Firefly Luciferase in Reporter Gene Assays

• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process • High Sensitivity: Wider linear range and robust luminescence signal and without DTT • The luminescence signal is not only high but also robust, with a half-life of approximately 1 h. It is suitable for most assay formats

Size: 10 mL2*50 mL2*250 mL

CAT.: A2000600N

Delivery: Available in Stock

Price: $ 125

Overview

KeyTec® Enhanced Luciferase Detection Kit (no DTT) is designed for the sensitive and robust detection of Firefly luciferase reporter gene assays without Dithiothreitol. Simply mix the substrate with cell lysis buffer, add the mixture to the cells, and proceed to detect the Firefly luciferase signal within the cells.
The detection principle is based on Luminescent technology. Within the kit, D-Luciferin reacts with Firefly luciferase released by cells, generating a sensitive and robust luminescence signal. The process provides a highly sensitive, robust, and homogeneous assay for the detection of Firefly luciferase reporter gene expression in mammalian cells.

Performance

Use KeyTec® Enhanced Luciferase Detection Kit (no DTT) to detect the overexpression of Firefly luciferase in the HEK293 cell line. The results showed a linear relationship between the luminescence signal and the number of cells.  HEK293 cells, cultured in DMEM medium with 10% FBS, were serially diluted two-fold, starting from 50,000 cells per well in a 96-well plate. Perform the assay according to the procedure outlined in “Assay Procedure”. Five minutes after adding the reagent, measure the luminescence signal using the Envision's Luminescence program. (Example program details include Mirror: Luminescence, Em filter: Luminescence 700, Measurement height: 6.5 mm, and Measurement time: 1 s).

Protocols

Protocol_A2000600N ∕ A2000601N ∕ A2000602N_KeyTec® Enhanced Luciferase Detection Kit (no DTT)_VKEYBIO-01-2024.pdf

Components

CAT.

Description

Size

A2000600N

KeyTec® Enhanced Luciferase Detection Kit (no DTT)

10 mL

Each kit contains sufficient reagents to perform 100 tests of 100 μL/well. 
The kit contains the following components:
       •  1 × 200 μL KeyTec® Enhanced Luciferase Substrate 50X (no DTT)
       •  1 × 10 mL KeyTec® Enhanced Luciferase Cell lysis buffer (no DTT)

Notices

Certificate of Analysis

Limitations

Storage Conditions

For research use only

-40 ℃

Related Products

Overview

KeyTec® Enhanced Luciferase Detection Kit (no DTT) is designed for the sensitive and robust detection of Firefly luciferase reporter gene assays without Dithiothreitol. Simply mix the substrate with cell lysis buffer, add the mixture to the cells, and proceed to detect the Firefly luciferase signal within the cells.
The detection principle is based on Luminescent technology. Within the kit, D-Luciferin reacts with Firefly luciferase released by cells, generating a sensitive and robust luminescence signal. The process provides a highly sensitive, robust, and homogeneous assay for the detection of Firefly luciferase reporter gene expression in mammalian cells.

Performance

Use KeyTec® Enhanced Luciferase Detection Kit (no DTT) to detect the overexpression of Firefly luciferase in the HEK293 cell line. The results showed a linear relationship between the luminescence signal and the number of cells.  HEK293 cells, cultured in DMEM medium with 10% FBS, were serially diluted two-fold, starting from 50,000 cells per well in a 96-well plate. Perform the assay according to the procedure outlined in “Assay Procedure”. Five minutes after adding the reagent, measure the luminescence signal using the Envision's Luminescence program. (Example program details include Mirror: Luminescence, Em filter: Luminescence 700, Measurement height: 6.5 mm, and Measurement time: 1 s).

Protocols

Protocol_A2000600N ∕ A2000601N ∕ A2000602N_KeyTec® Enhanced Luciferase Detection Kit (no DTT)_VKEYBIO-01-2024.pdf

Components

CAT.

Description

Size

A2000600N

KeyTec® Enhanced Luciferase Detection Kit (no DTT)

10 mL

Each kit contains sufficient reagents to perform 100 tests of 100 μL/well. 
The kit contains the following components:
       •  1 × 200 μL KeyTec® Enhanced Luciferase Substrate 50X (no DTT)
       •  1 × 10 mL KeyTec® Enhanced Luciferase Cell lysis buffer (no DTT)

Notices

Certificate of Analysis

Limitations

Storage Conditions

For research use only

-40 ℃