KeyTec® FGFR4[V550L], N-GST

Used for Developing FGFR4[V550L] Biochemical Activity Assay or Binding Assay Models
•  Strict quality control: Each batch comes with a rigorous QC report
•  High activity: Each batch is activity-verified, providing high-quality protein
•  Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Size: 10 μg100 μg

Price: $ 945

CAT.: P1HI0062S

Delivery: 2-3 weeks

KeyTec® FGFR4[V550L], N-GST

Used for Developing FGFR4[V550L] Biochemical Activity Assay or Binding Assay Models

• Strict quality control: Each batch comes with a rigorous QC report • High activity: Each batch is activity-verified, providing high-quality protein • Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Size: 10 μg100 μg

CAT.: P1HI0062S

Delivery: 2-3 weeks

Price: $ 945

Overview

KeyTec® FGFR4[V555L], N-GST recombinant protein with N-terminal GST tag + TEV cleavage site was purified by GST affinity chromatography. The FGFR4[V555L] protein showed high activity in TR-FRET assay.
AA Sequences：	Uniprot: P22455-1, W460-T802(end), V550L
Tag：		N-terminal GST tag
Molecular Weight：		64.6 kDa
Species：	Human
Expression Host：	Sf9
Protein Concentration：	0.83 mg/mL by OD280
Purity：> 90% by SDS-PAGE
Form：	Liquid
Formulation：	50 mM Tris, 150 mM NaCl, 1 mM DTT, 20 mM reduced glutathione, 10% glycerol, 0.05% Brij35, pH7.5

Performance

The FGFR4[V555L] activity was detected using TR-FRET technology. The reaction was performed by incubating the FGFR4[V555L] protein, ATP and substrate at 25 ℃ for 60 min, adding the TR-FRET reagents at 25 ℃ for 60 min, then reading ratio 665/620 by Microplate Reader with TR-FRET.

Protocols

COA_P1HI0062_G130712011_EN.pdf

Components

CAT.

Description

Size

P1HI0062S

KeyTec® FGFR4[V550L], N-GST

10 μg

Notices

Certificate of Analysis

Limitations

Storage Conditions

For research use only

-80 ℃

Related Products

Overview

KeyTec® FGFR4[V555L], N-GST recombinant protein with N-terminal GST tag + TEV cleavage site was purified by GST affinity chromatography. The FGFR4[V555L] protein showed high activity in TR-FRET assay.
AA Sequences：	Uniprot: P22455-1, W460-T802(end), V550L
Tag：		N-terminal GST tag
Molecular Weight：		64.6 kDa
Species：	Human
Expression Host：	Sf9
Protein Concentration：	0.83 mg/mL by OD280
Purity：> 90% by SDS-PAGE
Form：	Liquid
Formulation：	50 mM Tris, 150 mM NaCl, 1 mM DTT, 20 mM reduced glutathione, 10% glycerol, 0.05% Brij35, pH7.5

Performance

The FGFR4[V555L] activity was detected using TR-FRET technology. The reaction was performed by incubating the FGFR4[V555L] protein, ATP and substrate at 25 ℃ for 60 min, adding the TR-FRET reagents at 25 ℃ for 60 min, then reading ratio 665/620 by Microplate Reader with TR-FRET.

Protocols

COA_P1HI0062_G130712011_EN.pdf

Components

CAT.

Description

Size

P1HI0062S

KeyTec® FGFR4[V550L], N-GST

10 μg

Notices

Certificate of Analysis

Limitations