KeyTec® GAK, N-His
Used for Developing GAK Biochemical Activity Assay or Binding Assay Models
• Strict quality control: Each batch comes with a rigorous QC report • High activity: Each batch is activity-verified, providing high-quality protein • Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
Size:
10 μg100 μg
Price: $ 660
CAT.: P1HE0063S
Delivery: 2-3 weeks
KeyTec® GAK, N-His
Used for Developing GAK Biochemical Activity Assay or Binding Assay Models
• Strict quality control: Each batch comes with a rigorous QC report • High activity: Each batch is activity-verified, providing high-quality protein • Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
Size:
10 μg100 μg
CAT.: P1HE0063S
Delivery: 2-3 weeks
Price: $ 660
OverviewKeyTec® GAK, N-His recombinant protein with N-terminal 6xHis tag was purified by Ni-NTA affinity and followed by SEC chromatography. The SEC-HPLC result showed that the GAK protein was monomer in the solution. The GAK protein showed high activity in ADP-Glo assay.
AA Sequences： Uniprot: O14976;A12-P347
Tag： N-terminal 6xHis tag
Molecular Weight： 39.8 kDa
Species： Human
Expression Host： E.coli
Protein Concentration： 0.88 mg/mL by OD280
Purity：> 95% by SDS-PAGE
Form： Liquid
Formulation： 20 mM Tris, 200 mM NaCl, 1 mM DTT, 10% glycerol, pH 7.5
Performance
The GAK activity was detected using ADP-Glo technology. The reaction was performed by incubating the GAK protein, ATP and substrate at 25 ℃ for 120 min, then reading RLU by Microplate Reader with Luminescence.
Protocols
COA_P1HE0063_A120720021_EN.pdf
ComponentsCAT.
Description
Size
P1HE0063S
KeyTec® GAK, N-His
10 μg
NoticesCertificate of Analysis
Limitations
Storage Conditions
For research use only
-80 ℃
Related ProductsOverviewKeyTec® GAK, N-His recombinant protein with N-terminal 6xHis tag was purified by Ni-NTA affinity and followed by SEC chromatography. The SEC-HPLC result showed that the GAK protein was monomer in the solution. The GAK protein showed high activity in ADP-Glo assay.
AA Sequences： Uniprot: O14976;A12-P347
Tag： N-terminal 6xHis tag
Molecular Weight： 39.8 kDa
Species： Human
Expression Host： E.coli
Protein Concentration： 0.88 mg/mL by OD280
Purity：> 95% by SDS-PAGE
Form： Liquid
Formulation： 20 mM Tris, 200 mM NaCl, 1 mM DTT, 10% glycerol, pH 7.5
PerformanceThe GAK activity was detected using ADP-Glo technology. The reaction was performed by incubating the GAK protein, ATP and substrate at 25 ℃ for 120 min, then reading RLU by Microplate Reader with Luminescence.
ProtocolsCOA_P1HE0063_A120720021_EN.pdf
ComponentsCAT.
Description
Size
P1HE0063S
KeyTec® GAK, N-His
10 μg
NoticesCertificate of Analysis
Limitations
Storage Conditions
For research use only
-80 ℃
Related Products沪ICP备2021012748号
WeChat
Sales Assistant:
Technical-Support:
WeChat Official Account:
Contact Us
Technical Support Email: technical-support@vkeybio.com
BD Email: business@vkeybio.com
Tel:
021-5835-6939
Cooperation