KeyTec® MAP2K1, N-His
Used for Developing MAP2K1 Biochemical Activity Assay or Binding Assay Models
• Strict quality control: Each batch comes with a rigorous QC report • High activity: Each batch is activity-verified, providing high-quality protein • Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
Size:
10 μg100 μg
Price: $ 660
CAT.: P1HI0071S
Delivery: 2-3 weeks
KeyTec® MAP2K1, N-His
Used for Developing MAP2K1 Biochemical Activity Assay or Binding Assay Models
• Strict quality control: Each batch comes with a rigorous QC report • High activity: Each batch is activity-verified, providing high-quality protein • Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
Size:
10 μg100 μg
CAT.: P1HI0071S
Delivery: 2-3 weeks
Price: $ 660
OverviewKeyTec® MAP2K1, N-His recombinant protein with N-terminal 8xHis tag + TEV clevage site was purified by Ni-NTA affinity chromatography. The MAP2K1 protein showed high activity in ADP-Glo assay.
AA Sequences： Uniprot: Q02750-1, M1-V393(end)
Tag： N-terminal 8xHis tag
Molecular Weight： 45.9 kDa
Species： Human
Expression Host： Sf9
Protein Concentration： 0.74 mg/mL by OD280
Purity：> 95% by SDS-PAGE
Form： Liquid
Formulation： 20 mM Hepes, 300 mM NaCl, 1 mM DTT, 0.05% Brij-35, 10% glycerol, pH7.5
Performance
The MAP2K1 activity was detected using ADP-Glo technology. The reaction was performed by incubating the MAP2K1 protein, ATP and substrate at 25 ℃ for 60 min, then reading RLU by Microplate Reader with Luminescence.
Protocols
COA_P1HI0071_K130425011_EN.pdf
ComponentsCAT.
Description
Size
P1HI0071S
KeyTec® MAP2K1, N-His
10 μg
NoticesCertificate of Analysis
Limitations
Storage Conditions
For research use only
-80 ℃
Related ProductsOverviewKeyTec® MAP2K1, N-His recombinant protein with N-terminal 8xHis tag + TEV clevage site was purified by Ni-NTA affinity chromatography. The MAP2K1 protein showed high activity in ADP-Glo assay.
AA Sequences： Uniprot: Q02750-1, M1-V393(end)
Tag： N-terminal 8xHis tag
Molecular Weight： 45.9 kDa
Species： Human
Expression Host： Sf9
Protein Concentration： 0.74 mg/mL by OD280
Purity：> 95% by SDS-PAGE
Form： Liquid
Formulation： 20 mM Hepes, 300 mM NaCl, 1 mM DTT, 0.05% Brij-35, 10% glycerol, pH7.5
PerformanceThe MAP2K1 activity was detected using ADP-Glo technology. The reaction was performed by incubating the MAP2K1 protein, ATP and substrate at 25 ℃ for 60 min, then reading RLU by Microplate Reader with Luminescence.
ProtocolsCOA_P1HI0071_K130425011_EN.pdf
ComponentsCAT.
Description
Size
P1HI0071S
KeyTec® MAP2K1, N-His
10 μg
NoticesCertificate of Analysis
Limitations
Storage Conditions
For research use only
-80 ℃
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