KeyTec® PLK1, N-GST

Used for Developing PLK1 Biochemical Activity Assay or Binding Assay Models
•  Strict quality control: Each batch comes with a rigorous QC report
•  High activity: Each batch is activity-verified, providing high-quality protein
•  Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Size: 10 μg100 μg

Price: $ 660

CAT.: P1HI0079S

Delivery: 2-3 weeks

KeyTec® PLK1, N-GST

Used for Developing PLK1 Biochemical Activity Assay or Binding Assay Models

• Strict quality control: Each batch comes with a rigorous QC report • High activity: Each batch is activity-verified, providing high-quality protein • Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Size: 10 μg100 μg

CAT.: P1HI0079S

Delivery: 2-3 weeks

Price: $ 660

Overview

KeyTec® PLK1, N-GST recombinant protein with N-terminal GST tag+TEV cleavage site was purified by GST affinity chromatography. The PLK1 protein showed high activity in ADP-Glo assay.
AA Sequences：	Uniprot: P53350, M1-S603(end)
Tag：		N-terminal GST tag
Molecular Weight：		94.9 kDa
Species：	Human
Expression Host：	Sf9
Protein Concentration：	0.55 mg/mL by OD280
Purity：> 60% by SDS-PAGE
Form：	Liquid
Formulation：	50 mM Tris, 150 mM NaCl, 10 mM Reduced Glu, 1 mM DTT, 10% glycerol, pH7.5

Performance

The PLK1 activity was detected using ADP-Glo technology. The reaction was performed by incubating the PLK1 protein, ATP and substrate at 25 ℃ for 60 min, adding the TR-FRET reagents at 25 ℃ for 60 min, then reading RLU by Microplate Reader with Luminescence.

Protocols

COA_P1HI0079_K130316011_EN.pdf

Components

CAT.

Description

Size

P1HI0079S

KeyTec® PLK1, N-GST

10 μg

Notices

Certificate of Analysis

Limitations

Storage Conditions

For research use only

-80 ℃

Related Products

Overview

KeyTec® PLK1, N-GST recombinant protein with N-terminal GST tag+TEV cleavage site was purified by GST affinity chromatography. The PLK1 protein showed high activity in ADP-Glo assay.
AA Sequences：	Uniprot: P53350, M1-S603(end)
Tag：		N-terminal GST tag
Molecular Weight：		94.9 kDa
Species：	Human
Expression Host：	Sf9
Protein Concentration：	0.55 mg/mL by OD280
Purity：> 60% by SDS-PAGE
Form：	Liquid
Formulation：	50 mM Tris, 150 mM NaCl, 10 mM Reduced Glu, 1 mM DTT, 10% glycerol, pH7.5

Performance

The PLK1 activity was detected using ADP-Glo technology. The reaction was performed by incubating the PLK1 protein, ATP and substrate at 25 ℃ for 60 min, adding the TR-FRET reagents at 25 ℃ for 60 min, then reading RLU by Microplate Reader with Luminescence.

Protocols

COA_P1HI0079_K130316011_EN.pdf

Components

CAT.

Description

Size

P1HI0079S

KeyTec® PLK1, N-GST

10 μg

Notices

Certificate of Analysis

Limitations