KeyTec® ROCK1, N-GST
Used for Developing ROCK1 Biochemical Activity Assay or Binding Assay Models
• Strict quality control: Each batch comes with a rigorous QC report • High activity: Each batch is activity-verified, providing high-quality protein • Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
Size:
10 μg100 μg
Price: $ 660
CAT.: P1HI0090S
Delivery: 2-3 weeks
KeyTec® ROCK1, N-GST
Used for Developing ROCK1 Biochemical Activity Assay or Binding Assay Models
• Strict quality control: Each batch comes with a rigorous QC report • High activity: Each batch is activity-verified, providing high-quality protein • Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
Size:
10 μg100 μg
CAT.: P1HI0090S
Delivery: 2-3 weeks
Price: $ 660
OverviewKeyTec® ROCK1, N-GST recombinant protein with N-terminal GST tag was purified by GST affinity chromatography. The ROCK1 protein showed high activity in TR-FRET assay.
AA Sequences： Uniprot: Q13464, M1-L535
Tag： N-terminal GST tag
Molecular Weight： 87.9 kDa
Species： Human
Expression Host： Sf9
Protein Concentration： 0.80 mg/mL by OD280
Purity：>90% by SDS-PAGE
Form： Liquid
Formulation： 20 mM Hepes, 300 mM NaCl, 20 mM Reduced Glu, 10% glycerol, pH7.0
Performance
The ROCK1 activity was detected using TR-FRET technology. The reaction was performed by incubating the ROCK1 protein, ATP and substrate at 25 ℃ for 60 min, adding the TR-FRET reagents at 25 ℃ for 60 min, then reading ratio 665/620 by Microplate Reader with TR-FRET.
Protocols
COA_P1HI0090_K130223011_EN.pdf
ComponentsCAT.
Description
Size
P1HI0090S
KeyTec® ROCK1, N-GST
10 μg
NoticesCertificate of Analysis
Limitations
Storage Conditions
For research use only
-80 ℃
Related ProductsOverviewKeyTec® ROCK1, N-GST recombinant protein with N-terminal GST tag was purified by GST affinity chromatography. The ROCK1 protein showed high activity in TR-FRET assay.
AA Sequences： Uniprot: Q13464, M1-L535
Tag： N-terminal GST tag
Molecular Weight： 87.9 kDa
Species： Human
Expression Host： Sf9
Protein Concentration： 0.80 mg/mL by OD280
Purity：>90% by SDS-PAGE
Form： Liquid
Formulation： 20 mM Hepes, 300 mM NaCl, 20 mM Reduced Glu, 10% glycerol, pH7.0
PerformanceThe ROCK1 activity was detected using TR-FRET technology. The reaction was performed by incubating the ROCK1 protein, ATP and substrate at 25 ℃ for 60 min, adding the TR-FRET reagents at 25 ℃ for 60 min, then reading ratio 665/620 by Microplate Reader with TR-FRET.
ProtocolsCOA_P1HI0090_K130223011_EN.pdf
ComponentsCAT.
Description
Size
P1HI0090S
KeyTec® ROCK1, N-GST
10 μg
NoticesCertificate of Analysis
Limitations
Storage Conditions
For research use only
-80 ℃
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