KeyTec® Renilla Luciferase Detection Kit

Luminescent Assay for Detecting Renilla Luciferase in Reporter Gene Assays
•  Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
•  High Sensitivity: Wider linear range and low background
•  No need for separate lysis, and the luminescence signal is not only high but also robust, with a half-life of approximately 1-2 h

Size: 10 mL2*50 mL2*250 mL

Price: $ 125

CAT.: A2001100N

Delivery: Available in Stock

KeyTec® Renilla Luciferase Detection Kit

Luminescent Assay for Detecting Renilla Luciferase in Reporter Gene Assays

• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process • High Sensitivity: Wider linear range and low background • No need for separate lysis, and the luminescence signal is not only high but also robust, with a half-life of approximately 1-2 h

Size: 10 mL2*50 mL2*250 mL

CAT.: A2001100N

Delivery: Available in Stock

Price: $ 125

Overview

KeyTec® Renilla luciferase Detection kit is designed for the sensitive and robust detection of Renilla luciferase reporter gene assays. Simply mix the substrate with cell lysis buffer, add the mixture to the cells, and proceed to detect the Renilla luciferase signal within the cells.
The detection principle is based on Luminescent technology. Within the kit, Coelenterazine reacts with Renilla luciferase released by cells, generating a sensitive and robust luminescence signal. The process provides a highly sensitive, robust, and homogeneous assay for the detection of Renilla luciferase reporter gene expression in mammalian cells. 

Performance

Use KeyTec® Renilla Luciferase Detection Kit to detect the overexpression of Renilla luciferase in the HEK293 cell line . The results showed a linear relationship between the luminescence signal and the number of cells.  HEK293 cells, cultured in DMEM medium with 10% FBS, were serially diluted two-fold, starting from 50,000 cells per well in a 96-well plate. Perform the assay according to the procedure outlined in “Assay Procedure”. Five minutes after adding the reagent, measure the luminescence signal using the Envision's Luminescence program. (Example program details include Mirror: Luminescence, Em filter: Luminescence 700, Measurement height: 6.5 mm, and Measurement time: 1 s).

Protocols

Protocol_A2001100N ∕ A2001101N ∕ A2001102N_KeyTec® Renilla Luciferase Detection Kit_VKEYBIO-01-2024.pdf

Components

CAT.

Description

Size

A2001100N

KeyTec® Renilla Luciferase Detection Kit

10 mL

Each kit contains sufficient reagents to perform 100 tests of 100 μL/well. 
The kit contains the following components:
       •  1 × 100 μL KeyTec® Renilla Luciferase Substrate 100X
       •  1 × 10 mL KeyTec® Renilla Luciferase Cell lysis buffer

Notices

Certificate of Analysis

Limitations

Storage Conditions

For research use only

-20 ℃

Related Products

Overview

KeyTec® Renilla luciferase Detection kit is designed for the sensitive and robust detection of Renilla luciferase reporter gene assays. Simply mix the substrate with cell lysis buffer, add the mixture to the cells, and proceed to detect the Renilla luciferase signal within the cells.
The detection principle is based on Luminescent technology. Within the kit, Coelenterazine reacts with Renilla luciferase released by cells, generating a sensitive and robust luminescence signal. The process provides a highly sensitive, robust, and homogeneous assay for the detection of Renilla luciferase reporter gene expression in mammalian cells. 

Performance

Use KeyTec® Renilla Luciferase Detection Kit to detect the overexpression of Renilla luciferase in the HEK293 cell line . The results showed a linear relationship between the luminescence signal and the number of cells.  HEK293 cells, cultured in DMEM medium with 10% FBS, were serially diluted two-fold, starting from 50,000 cells per well in a 96-well plate. Perform the assay according to the procedure outlined in “Assay Procedure”. Five minutes after adding the reagent, measure the luminescence signal using the Envision's Luminescence program. (Example program details include Mirror: Luminescence, Em filter: Luminescence 700, Measurement height: 6.5 mm, and Measurement time: 1 s).

Protocols

Protocol_A2001100N ∕ A2001101N ∕ A2001102N_KeyTec® Renilla Luciferase Detection Kit_VKEYBIO-01-2024.pdf

Components

CAT.

Description

Size

A2001100N

KeyTec® Renilla Luciferase Detection Kit

10 mL

Each kit contains sufficient reagents to perform 100 tests of 100 μL/well. 
The kit contains the following components:
       •  1 × 100 μL KeyTec® Renilla Luciferase Substrate 100X
       •  1 × 10 mL KeyTec® Renilla Luciferase Cell lysis buffer

Notices

Certificate of Analysis

Limitations

Storage Conditions

For research use only

-20 ℃