KeyTec® Src, N-GST
Used for Developing Src Biochemical Activity Assay or Binding Assay Models
• Strict quality control: Each batch comes with a rigorous QC report • High activity: Each batch is activity-verified, providing high-quality protein • Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
Size:
10 μg100 μg
Price: $ 660
CAT.: P1HI0091S
Delivery: 2-3 weeks
KeyTec® Src, N-GST
Used for Developing Src Biochemical Activity Assay or Binding Assay Models
• Strict quality control: Each batch comes with a rigorous QC report • High activity: Each batch is activity-verified, providing high-quality protein • Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
Size:
10 μg100 μg
CAT.: P1HI0091S
Delivery: 2-3 weeks
Price: $ 660
OverviewKeyTec® Src, N-GST recombinant protein with N-terminal GST tag+TEV cleavage site was purified by GST affinity chromatography. The Src protein showed high activity in TR-FRET assay.
AA Sequences： Uniprot: P12931-1, M1-L536 (end)
Tag： N-terminal GST-tag
Molecular Weight： 86.5 kDa
Species： Human
Expression Host： Sf9
Protein Concentration： 0.36 mg/mL by OD280
Purity：> 95% by SDS-PAGE
Form： Liquid
Formulation： 50 mM Tris, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
Performance
The Src activity was detected using TR-FRET technology. The reaction was performed by incubating the Src protein, ATP and substrate at 25 ℃ for 60 min, adding the TR-FRET reagents at 25 ℃ for 60 min, then reading ratio 665/620 by Microplate Reader with TR-FRET.
Protocols
COA_P1HI0091_K130720011_EN.pdf
ComponentsCAT.
Description
Size
P1HI0091S
KeyTec® Src, N-GST
10 μg
NoticesCertificate of Analysis
Limitations
Storage Conditions
For research use only
-80 ℃
Related ProductsOverviewKeyTec® Src, N-GST recombinant protein with N-terminal GST tag+TEV cleavage site was purified by GST affinity chromatography. The Src protein showed high activity in TR-FRET assay.
AA Sequences： Uniprot: P12931-1, M1-L536 (end)
Tag： N-terminal GST-tag
Molecular Weight： 86.5 kDa
Species： Human
Expression Host： Sf9
Protein Concentration： 0.36 mg/mL by OD280
Purity：> 95% by SDS-PAGE
Form： Liquid
Formulation： 50 mM Tris, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
PerformanceThe Src activity was detected using TR-FRET technology. The reaction was performed by incubating the Src protein, ATP and substrate at 25 ℃ for 60 min, adding the TR-FRET reagents at 25 ℃ for 60 min, then reading ratio 665/620 by Microplate Reader with TR-FRET.
ProtocolsCOA_P1HI0091_K130720011_EN.pdf
ComponentsCAT.
Description
Size
P1HI0091S
KeyTec® Src, N-GST
10 μg
NoticesCertificate of Analysis
Limitations
Storage Conditions
For research use only
-80 ℃
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