KeyTec® Src-Zap70, N-GST

Used for Developing Src-Zap70 Biochemical Activity Assay or Binding Assay Models
•  Strict quality control: Each batch comes with a rigorous QC report
•  High activity: Each batch is activity-verified, providing high-quality protein
•  Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Size: 10 μg100 μg

Price: $ 660

CAT.: P1HI0092S

Delivery: 2-3 weeks

KeyTec® Src-Zap70, N-GST

Used for Developing Src-Zap70 Biochemical Activity Assay or Binding Assay Models

• Strict quality control: Each batch comes with a rigorous QC report • High activity: Each batch is activity-verified, providing high-quality protein • Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Size: 10 μg100 μg

CAT.: P1HI0092S

Delivery: 2-3 weeks

Price: $ 660

Overview

KeyTec® Src-Zap70, N-GST recombinant protein with N-terminal GST tag+thrombin cleavage site was purified by GST affinity chromatography. The Src-Zap70 protein showed high activity in TR-FRET assay.
AA Sequences：	Uniprot: P12931-1, Q254-L536(end)
Tag：		N-terminal GST-tag, C-Zap70
Molecular Weight：		61.3 kDa
Species：	Human
Expression Host：	Sf9
Protein Concentration：	0.49 mg/mL by OD280
Purity：>50% by SDS-PAGE
Form：	Liquid
Formulation：	20 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5

Performance

The Src-Zap70 activity was detected using TR-FRET technology. The reaction was performed by incubating the Src-Zap70 protein, ATP and substrate at 25 ℃ for 120 min, adding the TR-FRET reagents at 25 ℃ for 60 min, then reading ratio 665/620 by Microplate Reader with TR-FRET.

Protocols

COA_P1HI0092_G130212022_EN.pdf

Components

CAT.

Description

Size

P1HI0092S

KeyTec® Src-Zap70, N-GST

10 μg

Notices

Certificate of Analysis

Limitations

Storage Conditions

For research use only

-80 ℃

Related Products

Overview

KeyTec® Src-Zap70, N-GST recombinant protein with N-terminal GST tag+thrombin cleavage site was purified by GST affinity chromatography. The Src-Zap70 protein showed high activity in TR-FRET assay.
AA Sequences：	Uniprot: P12931-1, Q254-L536(end)
Tag：		N-terminal GST-tag, C-Zap70
Molecular Weight：		61.3 kDa
Species：	Human
Expression Host：	Sf9
Protein Concentration：	0.49 mg/mL by OD280
Purity：>50% by SDS-PAGE
Form：	Liquid
Formulation：	20 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5

Performance

The Src-Zap70 activity was detected using TR-FRET technology. The reaction was performed by incubating the Src-Zap70 protein, ATP and substrate at 25 ℃ for 120 min, adding the TR-FRET reagents at 25 ℃ for 60 min, then reading ratio 665/620 by Microplate Reader with TR-FRET.

Protocols

COA_P1HI0092_G130212022_EN.pdf

Components

CAT.

Description

Size

P1HI0092S

KeyTec® Src-Zap70, N-GST

10 μg

Notices

Certificate of Analysis

Limitations