KeyTec® Stable Luciferase Detection Kit
Luminescent Assay for Detecting Firefly Luciferase in Reporter Gene Assays
• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process • Long half-life: Stable luminescence signal with 2-hour half-life • With the longest half-life in reporter gene assay detection kits, it's suitable for high-throughput screening and other applications that require an extended reading time
Size:
10 mL2*50 mL2*250 mL4*250 mL
Price: $ 86
CAT.: A2000200N
Delivery: Available in Stock
KeyTec® Stable Luciferase Detection Kit
Luminescent Assay for Detecting Firefly Luciferase in Reporter Gene Assays
• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process • Long half-life: Stable luminescence signal with 2-hour half-life • With the longest half-life in reporter gene assay detection kits, it's suitable for high-throughput screening and other applications that require an extended reading time
Size:
10 mL2*50 mL2*250 mL4*250 mL
CAT.: A2000200N
Delivery: Available in Stock
Price: $ 86
OverviewKeyTec® Stable luciferase Detection kit is designed for the exceedingly robust detection of Firefly luciferase reporter gene assays suitable for High Throughput Screening (HTS). Simply mix the substrate with cell lysis buffer, add the mixture to the cells, and proceed to detect the Firefly luciferase signal within the cells.
The detection principle is based on Luminescent technology. Within the kit, D-Luciferin reacts with Firefly luciferase released by cells, generating a long half-life luminescence signal. The process provides a high repeatability, robust, and homogeneous assay for the detection of Firefly luciferase reporter gene expression in mammalian cells.
PerformanceUse KeyTec® Stable Luciferase Detection Kit to detect the overexpression of Firefly luciferase in the HEK293 cell line. The results showed a linear relationship between the luminescence signal and the number of cells. HEK293 cells, cultured in DMEM medium with 10% FBS, were serially diluted two-fold, starting from 50,000 cells per well in a 96-well plate. Perform the assay according to the procedure outlined in “Assay Procedure”. Five minutes after adding the reagent, measure the luminescence signal using the Envision's Luminescence program. (Example program details include Mirror: Luminescence, Em filter: Luminescence 700, Measurement height: 6.5 mm, and Measurement time: 1 s).
Protocols
Protocol_A2000200N ∕ A2000201N ∕ A2000202N ∕ A2000203N_KeyTec® Stable Luciferase Detection Kit_VKEYBIO-01-2024.pdf
ComponentsCAT.
Description
Size
A2000200N
KeyTec® Stable Luciferase Detection Kit
10 mL
Each kit contains sufficient reagents to perform 100 tests of 100 μL/well.
The kit contains the following components:
• 1 × 200 μL KeyTec® Stable Luciferase Substrate 50X
• 1 × 10 mL KeyTec® Stable Luciferase Cell lysis buffer
NoticesCertificate of Analysis
Limitations
Storage Conditions
For research use only
-40 ℃
Related ProductsOverviewKeyTec® Stable luciferase Detection kit is designed for the exceedingly robust detection of Firefly luciferase reporter gene assays suitable for High Throughput Screening (HTS). Simply mix the substrate with cell lysis buffer, add the mixture to the cells, and proceed to detect the Firefly luciferase signal within the cells.
The detection principle is based on Luminescent technology. Within the kit, D-Luciferin reacts with Firefly luciferase released by cells, generating a long half-life luminescence signal. The process provides a high repeatability, robust, and homogeneous assay for the detection of Firefly luciferase reporter gene expression in mammalian cells.
PerformanceUse KeyTec® Stable Luciferase Detection Kit to detect the overexpression of Firefly luciferase in the HEK293 cell line. The results showed a linear relationship between the luminescence signal and the number of cells. HEK293 cells, cultured in DMEM medium with 10% FBS, were serially diluted two-fold, starting from 50,000 cells per well in a 96-well plate. Perform the assay according to the procedure outlined in “Assay Procedure”. Five minutes after adding the reagent, measure the luminescence signal using the Envision's Luminescence program. (Example program details include Mirror: Luminescence, Em filter: Luminescence 700, Measurement height: 6.5 mm, and Measurement time: 1 s).
ProtocolsProtocol_A2000200N ∕ A2000201N ∕ A2000202N ∕ A2000203N_KeyTec® Stable Luciferase Detection Kit_VKEYBIO-01-2024.pdf
ComponentsCAT.
Description
Size
A2000200N
KeyTec® Stable Luciferase Detection Kit
10 mL
Each kit contains sufficient reagents to perform 100 tests of 100 μL/well.
The kit contains the following components:
• 1 × 200 μL KeyTec® Stable Luciferase Substrate 50X
• 1 × 10 mL KeyTec® Stable Luciferase Cell lysis buffer
NoticesCertificate of Analysis
Limitations
Storage Conditions
For research use only
-40 ℃
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