KeyTec® TR-FRET GDP loaded KRAS[G12D]/RAF1 Binding Assay kit

TR-FRET Assay for measurement of the interaction between KRAS[G12D] and RAF1  proteins
•  Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
•  High Accuracy: Utilizes GDP-loaded KRAS[G12D] protein, aligning more closely with physiological conditions.
•  High S/B: The kit provides a superior window

Size: 1,000 tests10,000 tests

Price: $ 2,000

CAT.: A1090002S

Delivery: Available in Stock

KeyTec® TR-FRET GDP loaded KRAS[G12D]/RAF1 Binding Assay kit

TR-FRET Assay for measurement of the interaction between KRAS[G12D] and RAF1 proteins

• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process • High Accuracy: Utilizes GDP-loaded KRAS[G12D] protein, aligning more closely with physiological conditions. • High S/B: The kit provides a superior window

Size: 1,000 tests10,000 tests

CAT.: A1090002S

Delivery: Available in Stock

Price: $ 2,000

Overview

The KeyTec® TR-FRET GDP loaded KRAS[G12D]/RAF1 Binding Assay kit is designed for measurement of the interaction between KRAS[G12D] and RAF1 proteins. It is based on sandwich immunoassay model and utilizes TR-FRET technology, known for its ease of use, homogeneity (no wash), low background, high sensitivity, high accuracy.
The detection principle is based on TR-FRET technology. The Tag2-RAF1 protein binds to the donor (KeyTec® TR-FRET mAb anti-HIS-Solar Eu), and the Tag1-KRAS[G12D] [GDP loaded] protein binds to the acceptor (mAb anti-Tag1–LA). When KRAS[G12D] [GDP loaded] interacts with RAF1, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of protein interaction. Blocking this interaction with any compound, peptide, or antibody will reduce the TR-FRET signal.

Performance

 •The inhibitory effects of small molecules

 •The results of GTP EC50 

 •The results of  GDP IC50

Protocols

Protocol_A1090002_KeyTec® TR-FRET GDP loaded KRAS[G12D] ∕ RAF1 Binding Assay kit_VKEYBIO-01-2024.pdf

Components

CAT.

Description

Size

A1090002S

KeyTec® TR-FRET GDP loaded KRAS[G12D]/RAF1 Binding Assay kit

1,000 tests

The tests are sufficient in a 384-well microplate assay format, with 20 μL per well.
       •  1 × 45 µL Tag1-KRAS[G12D] [ GDP loaded ] (100X)
       •  1 × 45 µL Tag2-RAF1 (100X)
       •  1 × 50 µL mAb anti-Tag2–Solar Eu (100X)
       •  1 × 50 µL mAb anti-Tag1–LA (100X)
       •  1 × 45 µL GTP (100X，2.5 mM)
       •  1 × 50 mL Binding Assay Diluent Buffer
       •  1 × 30 mL Solar Eu Detection Buffer

Notices

Certificate of Analysis

Limitations

Storage Conditions

For research use only

-60℃ or blow

Related Products

Overview

The KeyTec® TR-FRET GDP loaded KRAS[G12D]/RAF1 Binding Assay kit is designed for measurement of the interaction between KRAS[G12D] and RAF1 proteins. It is based on sandwich immunoassay model and utilizes TR-FRET technology, known for its ease of use, homogeneity (no wash), low background, high sensitivity, high accuracy.
The detection principle is based on TR-FRET technology. The Tag2-RAF1 protein binds to the donor (KeyTec® TR-FRET mAb anti-HIS-Solar Eu), and the Tag1-KRAS[G12D] [GDP loaded] protein binds to the acceptor (mAb anti-Tag1–LA). When KRAS[G12D] [GDP loaded] interacts with RAF1, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of protein interaction. Blocking this interaction with any compound, peptide, or antibody will reduce the TR-FRET signal.

Performance

 •The inhibitory effects of small molecules

 •The results of GTP EC50 

 •The results of  GDP IC50

Protocols

Protocol_A1090002_KeyTec® TR-FRET GDP loaded KRAS[G12D] ∕ RAF1 Binding Assay kit_VKEYBIO-01-2024.pdf

Components

CAT.

Description

Size

A1090002S

KeyTec® TR-FRET GDP loaded KRAS[G12D]/RAF1 Binding Assay kit

1,000 tests

The tests are sufficient in a 384-well microplate assay format, with 20 μL per well.
       •  1 × 45 µL Tag1-KRAS[G12D] [ GDP loaded ] (100X)
       •  1 × 45 µL Tag2-RAF1 (100X)
       •  1 × 50 µL mAb anti-Tag2–Solar Eu (100X)
       •  1 × 50 µL mAb anti-Tag1–LA (100X)
       •  1 × 45 µL GTP (100X，2.5 mM)
       •  1 × 50 mL Binding Assay Diluent Buffer
       •  1 × 30 mL Solar Eu Detection Buffer

Notices

Certificate of Analysis

Limitations

Storage Conditions

For research use only

-60℃ or blow