KeyTec® TR-FRET Human Fc Detection kit

Size: 1,000 tests10,000 tests

Price: $ 1,484

CAT.: A1040002S

Delivery: Available in Stock

KeyTec® TR-FRET Human Fc Detection kit

Size: 1,000 tests10,000 tests

CAT.: A1040002S

Delivery: Available in Stock

Price: $ 1,484

Overview

The KeyTec® TR-FRET Human Fc detection kit is designed for quantitative measurement of human IgG or human Fc-tagged protein in supernatant. It is based on competitive immunoassay model and utilizes TR-FRET technology, known for its ease of use, homogeneity (no wash), low background, wide range, robustness.
The screening principle is based on TR-FRET technology. The mAb anti-hFc antibody is labeled with KeyTec® TR-FRET Solar Eu and Human IgG is labeled with KeyTec® TR-FRET LA, when mAb anti-hFc-Solar Eu binds to Human IgG-LA, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm. Native human IgG or human Fc-tagged protein competes with the Human IgG-LA for binding to anti-hFc antibodies. The specific signal is inversely proportional to the concentration of human IgG or human Fc-tagged protein in the standard or sample.

Performance

       •  Standard Curve
       •  Quantitative Range：50 ng/mL – 50, 000 ng/mL
       •  Incubation Condition：Incubate at room temperature for 2 hours

Protocols

Protocol_A1040002_KeyTec® TR-FRET Human Fc Detection kit_VKEYBIO-01-2024.pdf

Components

CAT.

Description

Size

A1040002S

KeyTec® TR-FRET Human Fc Detection kit

1,000 tests

The tests are sufficient in a 384-well microplate assay format, with 20 μL per well. 
       •  1 × 100 µL mAb anti-hFc - Solar Eu (50X)
       •  1 × 100 µL mAb Human IgG-LA (50X)
       •  1 × 150 µL Human IgG Standard (5 µg/mL)
       •  1 × 30 mL Biotherapeutics Diluent Buffer
       •  1 × 10 mL Biotherapeutics Detection Buffer

Notices

Certificate of Analysis

Limitations

Storage Conditions

For research use only

-60℃ or blow

Related Products

Overview

The KeyTec® TR-FRET Human Fc detection kit is designed for quantitative measurement of human IgG or human Fc-tagged protein in supernatant. It is based on competitive immunoassay model and utilizes TR-FRET technology, known for its ease of use, homogeneity (no wash), low background, wide range, robustness.
The screening principle is based on TR-FRET technology. The mAb anti-hFc antibody is labeled with KeyTec® TR-FRET Solar Eu and Human IgG is labeled with KeyTec® TR-FRET LA, when mAb anti-hFc-Solar Eu binds to Human IgG-LA, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm. Native human IgG or human Fc-tagged protein competes with the Human IgG-LA for binding to anti-hFc antibodies. The specific signal is inversely proportional to the concentration of human IgG or human Fc-tagged protein in the standard or sample.

Performance

       •  Standard Curve
       •  Quantitative Range：50 ng/mL – 50, 000 ng/mL
       •  Incubation Condition：Incubate at room temperature for 2 hours

Protocols

Protocol_A1040002_KeyTec® TR-FRET Human Fc Detection kit_VKEYBIO-01-2024.pdf

Components

CAT.

Description

Size

A1040002S

KeyTec® TR-FRET Human Fc Detection kit

1,000 tests

The tests are sufficient in a 384-well microplate assay format, with 20 μL per well. 
       •  1 × 100 µL mAb anti-hFc - Solar Eu (50X)
       •  1 × 100 µL mAb Human IgG-LA (50X)
       •  1 × 150 µL Human IgG Standard (5 µg/mL)
       •  1 × 30 mL Biotherapeutics Diluent Buffer
       •  1 × 10 mL Biotherapeutics Detection Buffer

Notices

Certificate of Analysis

Limitations

Storage Conditions

For research use only

-60℃ or blow