KeyTec® TR-FRET Human TNFα Detection kit
Sandwich Model TR-FRET Assay for Quantitative Measurement of Human TNFα Protein in Supernatant
• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process • High Sensitivity: A Wide linear range from 30 pg/mL to 5,000 pg/mL • Only 16 µL of the sample, no dilution required, highly suitable for HTS Assays
Size:
1,000 tests10,000 tests
Price: $ 2,102
CAT.: A1070001S
Delivery: Available in Stock
KeyTec® TR-FRET Human TNFα Detection kit
Sandwich Model TR-FRET Assay for Quantitative Measurement of Human TNFα Protein in Supernatant
• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process • High Sensitivity: A Wide linear range from 30 pg/mL to 5,000 pg/mL • Only 16 µL of the sample, no dilution required, highly suitable for HTS Assays
Size:
1,000 tests10,000 tests
CAT.: A1070001S
Delivery: Available in Stock
Price: $ 2,102
OverviewThe KeyTec® TR-FRET Human TNFαassay kit is designed for quantitative measurement of Human TNFα protein in supernatant. It is based on sandwich immunoassay model and utilizes TR-FRET technology, known for its ease of use, homogeneity (no wash), low background, high sensitivity, robustness.
The detection principle is based on TR-FRET technology. A pair of mAb anti-TNFα antibodies, each labeled with KeyTec® TR-FRET Solar Eu and KeyTec® TR-FRET LA, bind to the same antigen Human TNFα protein. This binding brings the donor molecule into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the concentration of Human TNFα protein.
Performance
• performance
• Quantitative Range：30 pg/mL – 5,000 pg/mL
• Incubation Condition： Incubate at room temperature for 3 hours
Protocols
Protocol_A1070001_KeyTec® TR-FRET Human TNFα Detection kit_VKEYBIO-02-2024.pdf
ComponentsCAT.
Description
Size
A1070001S
KeyTec® TR-FRET Human TNFα Detection kit
1,000 tests
The tests are sufficient in a 384-well microplate assay format, with 20 μL per well.
• 1 × 50 µL mAb anti-TNFα-Solar Eu (40X)
• 1 × 50 µL mAb anti-TNFα-LA (40X)
• 2 vials Lyophilized Human TNFα Standard
• 1 × 20 mL Cytokine Diluent Buffer
• 1 × 10 mL Cytokine Detection Buffer
NoticesCertificate of Analysis
Limitations
Storage Conditions
For research use only
-60℃ or blow
Related ProductsOverviewThe KeyTec® TR-FRET Human TNFαassay kit is designed for quantitative measurement of Human TNFα protein in supernatant. It is based on sandwich immunoassay model and utilizes TR-FRET technology, known for its ease of use, homogeneity (no wash), low background, high sensitivity, robustness.
The detection principle is based on TR-FRET technology. A pair of mAb anti-TNFα antibodies, each labeled with KeyTec® TR-FRET Solar Eu and KeyTec® TR-FRET LA, bind to the same antigen Human TNFα protein. This binding brings the donor molecule into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the concentration of Human TNFα protein.
Performance • performance
• Quantitative Range：30 pg/mL – 5,000 pg/mL
• Incubation Condition： Incubate at room temperature for 3 hours
ProtocolsProtocol_A1070001_KeyTec® TR-FRET Human TNFα Detection kit_VKEYBIO-02-2024.pdf
ComponentsCAT.
Description
Size
A1070001S
KeyTec® TR-FRET Human TNFα Detection kit
1,000 tests
The tests are sufficient in a 384-well microplate assay format, with 20 μL per well.
• 1 × 50 µL mAb anti-TNFα-Solar Eu (40X)
• 1 × 50 µL mAb anti-TNFα-LA (40X)
• 2 vials Lyophilized Human TNFα Standard
• 1 × 20 mL Cytokine Diluent Buffer
• 1 × 10 mL Cytokine Detection Buffer
NoticesCertificate of Analysis
Limitations
Storage Conditions
For research use only
-60℃ or blow
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