KeyTec® TR-FRET Hybridoma screening kit (Biotinylated antigen, Mouse IgG)

Size: 1,000 tests10,000 tests

Price: $ 1,824

CAT.: A1040006S

Delivery: Available in Stock

KeyTec® TR-FRET Hybridoma screening kit (Biotinylated antigen, Mouse IgG)

Size: 1,000 tests10,000 tests

CAT.: A1040006S

Delivery: Available in Stock

Price: $ 1,824

Overview

The KeyTec® TR-FRET Hybridoma screening kit (Biotinylated antigen, Mouse IgG) is designed for the simple and rapid screening of positive mouse hybridoma clones based on antigen-antibody affinity in cell supernatant. It is based on sandwich immunoassay model and utilizes TR-FRET technology, known for its ease of use, homogeneity (no wash), low background, high sensitivity, robustness.
The screening principle is based on TR-FRET technology. The Streptavidin-Solar Eu binds to the Biotinylated antigen, while the pAb anti-Mouse Fc-LA binds to the mouse Fc of IgG antibodies expressed by mouse hybridoma. When the antigen-antibody interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of protein interaction.

Performance

       •  Optimization of Antigen and Antibody Concentration
       •  Range of Antibody concentration: 100 ng/mL – 80 µg/mL*5
       •  Incubation Condition：Incubate at room temperature for 1 hours to overnight

       •  Effects of various matrices

Protocols

Protocol_A1040006_KeyTec® TR-FRET Hybridoma screening kit (Biotinylated antigen, Mouse IgG)_VKEYBIO-01-2024.pdf

Components

CAT.

Description

Size

A1040006S

KeyTec® TR-FRET Hybridoma screening kit (Biotinylated antigen, Mouse IgG)

1,000 tests

The tests are sufficient in a 384-well microplate assay format, with 20 μL per well. 
       •  1 × 100 µL Streptavidin - Solar Eu (50X)
       •  1 × 100 µL pAb anti-Mouse Fc - LA (50X)
       •  1 × 40 mL Hybridoma Detection Buffer
       •  1 × Positive Control (1X),Lyophilized

Notices

Certificate of Analysis

Limitations

Storage Conditions

For research use only

-60℃ or blow

Related Products

Overview

The KeyTec® TR-FRET Hybridoma screening kit (Biotinylated antigen, Mouse IgG) is designed for the simple and rapid screening of positive mouse hybridoma clones based on antigen-antibody affinity in cell supernatant. It is based on sandwich immunoassay model and utilizes TR-FRET technology, known for its ease of use, homogeneity (no wash), low background, high sensitivity, robustness.
The screening principle is based on TR-FRET technology. The Streptavidin-Solar Eu binds to the Biotinylated antigen, while the pAb anti-Mouse Fc-LA binds to the mouse Fc of IgG antibodies expressed by mouse hybridoma. When the antigen-antibody interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of protein interaction.

Performance

       •  Optimization of Antigen and Antibody Concentration
       •  Range of Antibody concentration: 100 ng/mL – 80 µg/mL*5
       •  Incubation Condition：Incubate at room temperature for 1 hours to overnight

       •  Effects of various matrices

Protocols

Protocol_A1040006_KeyTec® TR-FRET Hybridoma screening kit (Biotinylated antigen, Mouse IgG)_VKEYBIO-01-2024.pdf

Components

CAT.

Description

Size

A1040006S

KeyTec® TR-FRET Hybridoma screening kit (Biotinylated antigen, Mouse IgG)

1,000 tests

The tests are sufficient in a 384-well microplate assay format, with 20 μL per well. 
       •  1 × 100 µL Streptavidin - Solar Eu (50X)
       •  1 × 100 µL pAb anti-Mouse Fc - LA (50X)
       •  1 × 40 mL Hybridoma Detection Buffer
       •  1 × Positive Control (1X),Lyophilized

Notices

Certificate of Analysis

Limitations

Storage Conditions

For research use only

-60℃ or blow