KeyTec® TR-FRET Hybridoma screening kit (Human Fc antigen, Mouse IgG)
Size:
1,000 tests10,000 tests
Price: $ 1,824
CAT.: A1040005S
Delivery: Available in Stock
KeyTec® TR-FRET Hybridoma screening kit (Human Fc antigen, Mouse IgG)
Size:
1,000 tests10,000 tests
CAT.: A1040005S
Delivery: Available in Stock
Price: $ 1,824
OverviewThe KeyTec® TR-FRET Hybridoma screening kit (Human Fc antigen, Mouse IgG) is designed for the simple and rapid screening of positive mouse hybridoma clones based on antigen-antibody affinity in cell supernatant. It is based on sandwich immunoassay model and utilizes TR-FRET technology, known for its ease of use, homogeneity (no wash), low background, high sensitivity, robustness.
The screening principle is based on TR-FRET technology. The pAb anti hFc-Solar Eu binds to the Human Fc-tagged antigen, while the pAb anti-Mouse Fc-LA binds to the mouse Fc of IgG antibodies expressed by mouse hybridoma. When the antigen-antibody interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of protein interaction.
Performance
• Optimization of Antigen and Antibody Concentration
• Range of Antibody concentration: 80 ng/mL – 80 µg/mL*5
• Incubation Condition：Incubate at room temperature for 2 hours to overnight
• Effects of various matrices
Protocols
Protocol_A1040005_KeyTec® TR-FRET Hybridoma screening kit (Human Fc antigen, Mouse IgG)_VKEYBIO-01-2024.pdf
ComponentsCAT.
Description
Size
A1040005S
KeyTec® TR-FRET Hybridoma screening kit (Human Fc antigen, Mouse IgG)
1,000 tests
The tests are sufficient in a 384-well microplate assay format, with 20 μL per well.
• 1 × 100 µL mAb anti hFc - Solar Eu (50X)
• 1 × 100 µL pAb anti-Mouse Fc - LA (50X)
• 1 × 40 mL Hybridoma Detection Buffer
• 1 × Positive Control (1X),Lyophilized
NoticesCertificate of Analysis
Limitations
Storage Conditions
For research use only
-60℃ or blow
Related ProductsOverviewThe KeyTec® TR-FRET Hybridoma screening kit (Human Fc antigen, Mouse IgG) is designed for the simple and rapid screening of positive mouse hybridoma clones based on antigen-antibody affinity in cell supernatant. It is based on sandwich immunoassay model and utilizes TR-FRET technology, known for its ease of use, homogeneity (no wash), low background, high sensitivity, robustness.
The screening principle is based on TR-FRET technology. The pAb anti hFc-Solar Eu binds to the Human Fc-tagged antigen, while the pAb anti-Mouse Fc-LA binds to the mouse Fc of IgG antibodies expressed by mouse hybridoma. When the antigen-antibody interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of protein interaction.
Performance • Optimization of Antigen and Antibody Concentration
• Range of Antibody concentration: 80 ng/mL – 80 µg/mL*5
• Incubation Condition：Incubate at room temperature for 2 hours to overnight
• Effects of various matrices
ProtocolsProtocol_A1040005_KeyTec® TR-FRET Hybridoma screening kit (Human Fc antigen, Mouse IgG)_VKEYBIO-01-2024.pdf
ComponentsCAT.
Description
Size
A1040005S
KeyTec® TR-FRET Hybridoma screening kit (Human Fc antigen, Mouse IgG)
1,000 tests
The tests are sufficient in a 384-well microplate assay format, with 20 μL per well.
• 1 × 100 µL mAb anti hFc - Solar Eu (50X)
• 1 × 100 µL pAb anti-Mouse Fc - LA (50X)
• 1 × 40 mL Hybridoma Detection Buffer
• 1 × Positive Control (1X),Lyophilized
NoticesCertificate of Analysis
Limitations
Storage Conditions
For research use only
-60℃ or blow
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