KeyTec® TR-FRET Protein A-Solar Eu (100X)

KeyTec® TR-FRET Protein A-Solar Eu is designed for developing the TR-FRET Assay. Protein A exhibits high affinity towards various species of Ig, particularly IgG. In the Antigen-Antibody Interaction assay, the IgG antibody binds to the donor (KeyTec® TR-FRET Protein A-Solar Eu*1), and the antigen is labeled (directly or indirectly) with the acceptor (KeyTec® TR-FRET LA/HX*2). When the Antigen-Antibody interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of antigen-antibody interaction.