KeyTec® TR-FRET pAb anti-Human IgG-Solar Eu is designed for developing the TR-FRET Assay. The anti-Human IgG antibody is a mouse polyclonal antibody. In the Antigen-Antibody Interaction assay, the Human IgG antibody binds to the donor (KeyTec® TR-FRET pAb anti-Human IgG-Solar Eu*1), and the antigen is labeled (directly or indirectly) with the acceptor (KeyTec® TR-FRET LA/HX*2). When the Antigen-Antibody interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of antigen-antibody interaction.