KeyTec® WEE1, N-GST
Used for Developing WEE1 Biochemical Activity Assay or Binding Assay Models
• Strict quality control: Each batch comes with a rigorous QC report • High activity: Each batch is activity-verified, providing high-quality protein • Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
Size:
10 μg100 μg
Price: $ 660
CAT.: P1HI0160S
Delivery: 2-3 weeks
KeyTec® WEE1, N-GST
Used for Developing WEE1 Biochemical Activity Assay or Binding Assay Models
• Strict quality control: Each batch comes with a rigorous QC report • High activity: Each batch is activity-verified, providing high-quality protein • Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
Size:
10 μg100 μg
CAT.: P1HI0160S
Delivery: 2-3 weeks
Price: $ 660
OverviewKeyTec® WEE1, N-GST recombinant protein with N-terminal GST tag was purified by GST affinity chromatography. The WEE1 protein showed high activity in ADP-Glo assay.
AA Sequences：Uniprot: P30291-1, K219-Y646(end)
Tag： N-terminal GST tag
Molecular Weight： 74.4 kDa
Species：Human
Expression Host：Sf9
Protein Concentration：0.38 mg/mL by OD280
Purity：>85% by SDS-PAGE
Form：Liquid
Formulation：50 mM Tris, 150 mM NaCl, 1 mM DTT, pH7.5
Performance
The WEE1 activity was detected using ADP-Glo technology. The reaction was performed by incubating the WEE1 protein, ATP and substrate at 25 ℃ for 60 min, then reading RLU by Microplate Reader with Luminescence.
Protocols
COA_P1HI0160_K131108011_EN.pdf
ComponentsCAT.
Description
Size
P1HI0160S
KeyTec® WEE1, N-GST
10 μg
NoticesCertificate of Analysis
Limitations
Storage Conditions
For research use only
-80 ℃
Related ProductsOverviewKeyTec® WEE1, N-GST recombinant protein with N-terminal GST tag was purified by GST affinity chromatography. The WEE1 protein showed high activity in ADP-Glo assay.
AA Sequences：Uniprot: P30291-1, K219-Y646(end)
Tag： N-terminal GST tag
Molecular Weight： 74.4 kDa
Species：Human
Expression Host：Sf9
Protein Concentration：0.38 mg/mL by OD280
Purity：>85% by SDS-PAGE
Form：Liquid
Formulation：50 mM Tris, 150 mM NaCl, 1 mM DTT, pH7.5
PerformanceThe WEE1 activity was detected using ADP-Glo technology. The reaction was performed by incubating the WEE1 protein, ATP and substrate at 25 ℃ for 60 min, then reading RLU by Microplate Reader with Luminescence.
ProtocolsCOA_P1HI0160_K131108011_EN.pdf
ComponentsCAT.
Description
Size
P1HI0160S
KeyTec® WEE1, N-GST
10 μg
NoticesCertificate of Analysis
Limitations
Storage Conditions
For research use only
-80 ℃
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