KeyTec® cAMP TR-FRET KIT

KeyTec® cAMP TR-FRET KIT designed for quantitative measurement of adenosine 3',5'-cyclic adenosine monophosphate (cAMP) in cell lysates. It is based on competitive immunoassay model and utilizes TR-FRET technology, known for its ease of use, homogeneity (no wash), low background, wide range, robustness. The detection principle is based on TR-FRET technology. Streptavidin and anti-cAMP antibodies, each labeled with KeyTec® TR-FRET Solar Eu and KeyTec® TR-FRET LA, Streptavidin-Solar Eu form a complex with Biotin–cAMP referred to as the cAMP tracer. When cAMP tracer binds to mAb anti-cAMP–LA, excitation of the donor will result in the generation of the TR-FRET signal at 665 nm. Native cAMP produced by cells or unlabeled cAMP (standard curve) competes with the cAMP tracer for binding to anti-cAMP antibodies. The specific signal is inversely proportional to the concentration of cAMP in the standard or sample.