KeyTec® AAK1, N-His (E.coli)
Size: Used for Developing AAK1 Biochemical Activity Assay or Binding Assay Models
Price: $ 660
CAT.: P1HE0001S
• Strict quality control: Each batch comes with a rigorous QC report
• High activity: Each batch is activity-verified, providing high-quality protein
• Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
• Strict quality control: Each batch comes with a rigorous QC report
• High activity: Each batch is activity-verified, providing high-quality protein
• Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening
KeyTec® AAK1, N-His (E.coli) recombinant protein with N-terminal 6xHis tag was purified by Ni-NTA affinity and followed by SEC chromatography. The SEC-HPLC result showed that the AAK1 protein was dimer and monomer in the solution. The AAK1 protein showed high activity in ADP-Glo assay.
AA Sequences：Uniprot: Q2M2I8-1;T27-A365
Tag：N-terminal 6xHis tag
Molecular Weight：40.2 kDa
Species：Human
Expression Host：E.coli
Protein Concentration：0.32 mg/mL by OD280
Purity：> 95% by SDS-PAGE
Form：Liquid
Formulation：20 mM Tris, 300 mM NaCl, 1 mM DTT, 10% glycerol, pH 7.5
The AAK1 activity was detected using ADP-Glo technology. The reaction was performed by incubating the AAK1 protein, ATP and substrate at 25 ℃ for 120 min, then reading RLU by Microplate Reader with Luminescence.
| CAT. | Description | Size |
| P1HE0001S | KeyTec® AAK1, N-His (E.coli) | 10 μg |
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-80 ℃ |
| CAT. | Description | Size |
| P1HE0001L | KeyTec® AAK1, N-His (E.coli) | 100 μg |
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-80 ℃ |
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