KeyTec® AAK1, N-His (E.coli)

Quick replication of product information

Used for Developing AAK1 Biochemical Activity Assay or Binding Assay Models

• Strict quality control: Each batch comes with a rigorous QC report
• High activity: Each batch is activity-verified, providing high-quality protein
• Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Used for Developing AAK1 Biochemical Activity Assay or Binding Assay Models

Size:

10 μg

100 μg

Price:

$ 660

CAT.:

P1HE0001S

Delivery:

2-3 weeks

Price:

$ 3433

CAT.:

P1HE0001L

Delivery:

2-3 weeks

KeyTec® AAK1, N-His (E.coli)

10 μg

100 μg

CAT.: P1HE0001S

$ 660 $ 3433

Overview

Performance

Protocols

Components

Notices

Overview

KeyTec® AAK1, N-His (E.coli) recombinant protein with N-terminal 6xHis tag was purified by Ni-NTA affinity and followed by SEC chromatography. The SEC-HPLC result showed that the AAK1 protein was dimer and monomer in the solution. The AAK1 protein showed high activity in ADP-Glo assay.
AA Sequences：Uniprot: Q2M2I8-1;T27-A365
Tag：N-terminal 6xHis tag
Molecular Weight：40.2 kDa
Species：Human
Expression Host：E.coli
Protein Concentration：0.32 mg/mL by OD280
Purity：> 95% by SDS-PAGE
Form：Liquid
Formulation：20 mM Tris, 300 mM NaCl, 1 mM DTT, 10% glycerol, pH 7.5

Performance

The AAK1 activity was detected using ADP-Glo technology. The reaction was performed by incubating the AAK1 protein, ATP and substrate at 25 ℃ for 120 min, then reading RLU by Microplate Reader with Luminescence.